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PMID: 3041380 Published · ppublish English Journal Article

Chimeric multispecific DNA methyltransferases with novel combinations of target recognition.

Nucleic acids research ·Vol. 16 ·No. 14A ·1988-07-25 ·Pages 6649-58

Trautner TA, Balganesh TS, Pawlek B

Abstract

DNA target recognizing domains of different multispecific DNA-cytosine-methyltransferases can be rearranged through engineering of the corresponding genes to generate enzymes with novel combinations of target recognition.

MeSH Terms
DNA (Cytosine-5-)-Methyltransferases/physiology DNA Mutational Analysis DNA-Binding Proteins/physiology Escherichia coli/enzymology Plasmids Recombinant Proteins Structure-Activity Relationship Substrate Specificity
Chemicals
DNA-Binding Proteins Recombinant Proteins DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Trautner T A
Max-Planck-Institut für Molekulare Genetik, Berlin, FRG.
Balganesh T S
Pawlek B
References (12)
12 references, click to expand
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  2. Escherichia coli K-12 restricts DNA containing 5-methylcytosine.
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    Mol Gen Genet. 1979 Jan 10;168(2):165-72 PMID: 109739
  9. Restriction and modification in Bacillus subtilis: nucleotide sequence, functional organization and product of the DNA methyltransferase gene of bacteriophage SPR.
    Gene. 1984 Jul-Aug;29(1-2):51-61 PMID: 6092231
  10. Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR.
    Nucleic Acids Res. 1984 Dec 11;12(23):9039-49 PMID: 6096817
  11. DNA methyltransferase genes of Bacillus subtilis phages: structural relatedness and gene expression.
    Gene. 1985;35(1-2):143-50 PMID: 3928442
  12. Construction and use of chimeric SPR/phi 3T DNA methyltransferases in the definition of sequence recognizing enzyme regions.
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.14.6649
Published
1988-07-25
Pages
6649-58
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338320
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.14.6649 PMC Full Text View journal details
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