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PMID: 3043374 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence-directed bends of DNA helix axis at the upstream activation sites of alpha-cell-specific genes in yeast.

Nucleic acids research ·Vol. 16 ·No. 14B ·1988-07-25 ·Pages 6693-711

Inokuchi K, Nakayama A, Hishinuma F

Abstract

The MF alpha 1 gene of Saccharomyces cerevisiae, a major structural gene for mating pheromone alpha factor, is an alpha-cell-specific gene whose expression is regulated by the mating-type locus, MAT. Two upstream activation sites (UASMF alpha 1s), which are binding sites for an activator protein, MAT alpha 1, mediate alpha-cell-specific expression of this gene. We show here that DNA fragments containing the UASMF alpha 1 region exhibited anomalous slow electrophoretic mobilities on gels at lower temperature, but not at higher temperature, that is characteristic of bent DNA. We confirmed the sequence-directed bend at the UASMF alpha 1 region by employing a circular permutation analysis and a DNA cyclization assay. Deletion analyses revealed the existence of two bends in this region, each of which overlaps each UASMF alpha 1 element. The two bends were almost in phase; they lie in a nearly same plane with a same direction. We also show the existence of sequence-directed bend at the UAS region of the STE3 gene, another alpha-specific gene in S. cerevisiae. These bent DNAs may be involved in transcriptional regulation of this set of genes.

MeSH Terms
Base Sequence DNA Mutational Analysis DNA, Fungal/genetics Genes, Fungal Genes, Mating Type, Fungal Mating Factor Molecular Sequence Data Nucleic Acid Conformation Peptides/genetics Promoter Regions, Genetic Regulatory Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Structure-Activity Relationship
Chemicals
DNA, Fungal Peptides Mating Factor
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Inokuchi K
Laboratory of Molecular Genetics, Mitsubishi Kasei Institute of Life Sciences, Tokyo, Japan.
Nakayama A
Hishinuma F
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36 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.14.6693
Published
1988-07-25
Pages
6693-711
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338326
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/16.14.6693 PMC Full Text View journal details
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