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PMID: 3044923 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Genetic control of intrachromosomal recombination in Saccharomyces cerevisiae. I. Isolation and genetic characterization of hyper-recombination mutations.

Genetics ·Vol. 119 ·No. 4 ·1988-08-00 ·Pages 779-90

Aguilera A, Klein HL

Abstract

Eight complementation groups have been defined for recessive mutations conferring an increased mitotic intrachromosomal recombination phenotype (hpr genes) in Saccharomyces cerevisiae. Some of the mutations preferentially increase intrachromosomal gene conversion (hpr4, hpr5 and hpr8) between repeated sequences, some increase loss of a marker between duplicated genes (hpr1 and hpr6), and some increase both types of events (hpr2, hpr3 and hpr7). New alleles of the CDC2 and CDC17 genes were recovered among these mutants. The mutants were also characterized for sensitivity to DNA damaging agents and for mutator activity. Among the more interesting mutants are hpr5, which shows a biased gene conversion in a leu2-112::URA3::leu2-k duplication; and hpr1, which has a much weaker effect on interchromosomal mitotic recombination than on intrachromosomal mitotic recombination. These analyses suggest that gene conversion and reciprocal exchange can be separated mutationally. Further studies are required to show whether different recombination pathways or different outcomes of the same recombination pathway are controlled by the genes identified in this study.

MeSH Terms
Cell Cycle Chromosomes/physiology DNA Repair Gene Conversion Mitosis Multigene Family Mutation Recombination, Genetic Saccharomyces cerevisiae/genetics
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Aguilera A
Department of Biochemistry, New York University, New York 10016.
Klein H L
References (49)
49 references, click to expand
  1. DNA sequences of frameshift and other mutations induced by ICR-170 in yeast.
    Genetics. 1985 Oct;111(2):233-41 PMID: 2414151
  2. Intrachromosomal gene conversion in yeast.
    Nature. 1981 Jan 15;289(5794):144-8 PMID: 7005693
  3. Analysis of meiosis-defective mutations in yeast by physical monitoring of recombination.
    Genetics. 1986 Jul;113(3):551-67 PMID: 3015718
  4. Suppressible and nonsuppressible +1 G-C base pair insertions induced by ICR-170 at the his4 locus in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Sep;5(9):2247-56 PMID: 2426578
  5. Meiotic exchange within and between chromosomes requires a common Rec function in Saccharomyces cerevisiae.
    Mol Cell Biol. 1985 Dec;5(12):3532-44 PMID: 3915779
  6. Two mechanisms for directional gene conversion.
    Proc Natl Acad Sci U S A. 1986 Oct;83(19):7386-90 PMID: 3463974
  7. Chromosomal translocations generated by high-frequency meiotic recombination between repeated yeast genes.
    Genetics. 1986 Nov;114(3):731-52 PMID: 3539696
  8. The RAD24 (= Rs1) gene product of Saccharomyces cerevisiae participates in two different pathways of DNA repair.
    Genetics. 1987 Jan;115(1):83-90 PMID: 3549445
  9. Meiotic gene conversion and crossing over between dispersed homologous sequences occurs frequently in Saccharomyces cerevisiae.
    Genetics. 1987 Feb;115(2):233-46 PMID: 3549449
  10. Meiotic recombination in yeast: alteration by multiple heterozygosities.
    Science. 1987 Sep 18;237(4821):1459-65 PMID: 2820060
  11. Intrachromosomal recombination in Saccharomyces cerevisiae: reciprocal exchange in an inverted repeat and associated gene conversion.
    Genetics. 1987 Dec;117(4):633-43 PMID: 2828154
  12. Spontaneous mitotic recombination in yeast: the hyper-recombinational rem1 mutations are alleles of the RAD3 gene.
    Genetics. 1988 Jun;119(2):289-301 PMID: 2840336
  13. Studies of gene mutation in Saccharomyces.
    Cold Spring Harb Symp Quant Biol. 1956;21:175-85 PMID: 13433590
  14. The effect of three rad genes on survival, inter- and intragenic mitotic recombination in Saccharomyces. I. UV irradiation without photoreactivation or liquid-holding post-treatment.
    Mol Gen Genet. 1975;136(1):75-86 PMID: 16094968
  15. Intergenic conversion and reiterated genes.
    Nature. 1981 Mar 19;290(5803):191-2 PMID: 7207612
  16. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  17. Gene conversion between duplicated genetic elements in yeast.
    Nature. 1981 Jul 23;292(5821):306-11 PMID: 6265790
  18. Retroviruses as mutagens: insertion and excision of a nontransforming provirus alter expression of a resident transforming provirus.
    Cell. 1981 Jul;25(1):23-36 PMID: 6268304
  19. The cyc1-11 mutation in yeast reverts by recombination with a nonallelic gene: composite genes determining the iso-cytochromes c.
    Proc Natl Acad Sci U S A. 1981 Oct;78(10):6334-8 PMID: 6273865
  20. Phenomenology and genetic control of mitotic recombination in yeast.
    Annu Rev Genet. 1981;15:57-89 PMID: 7039496
  21. Recombination between genes located on nonhomologous chromosomes in Saccharomyces cerevisiae.
    Genetics. 1982 Jul-Aug;101(3-4):369-404 PMID: 6757052
  22. Saccharomyces cerevisiae cdc2 mutants fail to replicate approximately one-third of their nuclear genome.
    Mol Cell Biol. 1983 Jun;3(6):1000-12 PMID: 6348512
  23. "A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity". Addendum.
    Anal Biochem. 1984 Feb;137(1):266-7 PMID: 6329026
  24. Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
    Mol Cell Biol. 1984 Jun;4(6):1020-34 PMID: 6330525
  25. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  26. A structure for amplified DNA.
    Cell. 1983 May;33(1):53-63 PMID: 6678612
  27. Lack of association between intrachromosomal gene conversion and reciprocal exchange.
    Nature. 1984 Aug 30-Sep 5;310(5980):748-53 PMID: 6088989
  28. Meiotic recombination between duplicated genetic elements in Saccharomyces cerevisiae.
    Genetics. 1985 Feb;109(2):303-32 PMID: 3882522
  29. Recombination in Saccharomyces cerevisiae: REC-gene mutants and DNA-binding proteins.
    Cold Spring Harb Symp Quant Biol. 1984;49:41-8 PMID: 6397298
  30. High-frequency meiotic gene conversion between repeated genes on nonhomologous chromosomes in yeast.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3350-4 PMID: 3889906
  31. Repair of alkylation damage: stability of methyl groups in Bacillus subtilis treated with methyl methanesulfonate.
    J Bacteriol. 1970 Jun;102(3):760-6 PMID: 4988041
  32. Genetic control of radiation sensitivity in Saccharomyces cerevisiae.
    Genetics. 1969 Jul;62(3):519-31 PMID: 5384484
  33. Properties of a supercoiled deoxyribonucleic acid-protein relaxation complex and strand specificity of the relaxation event.
    Biochemistry. 1970 Oct 27;9(22):4428-40 PMID: 4919547
  34. Conversion-associated recombination in yeast (hybrids-meiosis-tetrads-marker loci-models).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):101-5 PMID: 4550500
  35. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  36. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  37. Hyper-recombination in dam mutants of Escherichia coli K-12.
    Mol Gen Genet. 1976 Dec 22;149(3):273-7 PMID: 799245
  38. Method for the isolation of Escherichia coli mutants with enhanced recombination between chromosomal duplications.
    J Bacteriol. 1977 Apr;130(1):167-72 PMID: 323226
  39. Recombinant levels of Escherichia coli K-12 mutants deficient in various replication, recombination, or repair genes.
    J Bacteriol. 1978 Jun;134(3):958-66 PMID: 350859
  40. The influence of defects in excision and error prone repair on spontaneous and induced mitotic recombination and mutation in Saccharomyces cerevisiae.
    Mol Gen Genet. 1978 Apr 25;161(1):81-8 PMID: 353501
  41. CDC17: an essential gene that prevents telomere elongation in yeast.
    Cell. 1985 Aug;42(1):249-57 PMID: 3893744
  42. Altered fidelity of mitotic chromosome transmission in cell cycle mutants of S. cerevisiae.
    Genetics. 1985 Jul;110(3):381-95 PMID: 3894160
  43. Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase.
    Nature. 1978 Aug 31;274(5674):891-3 PMID: 355897
  44. Enhanced mitotic recombination in a ligase-defective mutant of the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4589-92 PMID: 388447
  45. The RAD52 gene is required for homothallic interconversion of mating types and spontaneous mitotic recombination in yeast.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):503-7 PMID: 6987653
  46. Recombination of dispersed repeated DNA sequences in yeast.
    Science. 1980 Sep 19;209(4463):1380-4 PMID: 6251545
  47. Mitotic recombination in yeast: isolation and characterization of mutants with enhanced spontaneous mitotic gene conversion rates.
    Genetics. 1980 Apr;94(4):825-39 PMID: 7002715
  48. Hyper-recombination in uvrD mutants of Escherichia coli K-12.
    Mol Gen Genet. 1980;180(1):185-91 PMID: 7003307
  49. Hyper-recombining recipient strains in bacterial conjugation.
    Genetics. 1986 May;113(1):13-33 PMID: 3519362
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/119.4.779
Published
1988-08-00
Pages
779-90
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1203464
Subset
IM
Grants
NIEHS NIH HHS · ES03847 · United States
NIGMS NIH HHS · GM30439 · United States
External Links
PubMed source DOI: 10.1093/genetics/119.4.779 PMC Full Text View journal details
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