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PMID: 3046753 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

The sequence specificity of homeodomain-DNA interaction.

Cell ·Vol. 54 ·No. 7 ·1988-09-23 ·Pages 1081-90

Desplan C, Theis J, O'Farrell PH

Abstract

The Drosophila developmental gene, engrailed, encodes a sequence-specific DNA binding activity. Using deletion constructs expressed as fusion proteins in E. coli, we localized this activity to the conserved homeodomain (HD). The binding site consensus, TCAATTAAAT, is found in clusters in the engrailed regulatory region. Weak binding of the En HD to one copy of a synthetic consensus is enhanced by adjacent copies. The distantly related HD encoded by fushi tarazu binds to the same sites as the En HD, but differs in its preference for related sites. Both HDs bind a second type of sequence, a repeat of TAA. The similarity in sequence specificity of En and Ftz HDs suggests that, within families of DNA binding proteins, close relatives will exhibit similar specificities. Competition among related regulatory proteins might govern which protein occupies a given binding site and consequently determine the ultimate effect of cis-acting regulatory sites.

MeSH Terms
Allosteric Regulation Animals Biological Evolution DNA-Binding Proteins/metabolism Deoxyribonucleases Drosophila/genetics Escherichia coli/genetics Genes, Regulator Immunologic Techniques Plasmids Recombinant Fusion Proteins/metabolism Repetitive Sequences, Nucleic Acid Sequence Homology, Nucleic Acid
Chemicals
DNA-Binding Proteins Recombinant Fusion Proteins Deoxyribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Desplan C
Department of Biochemistry and Biophysics, University of California, San Francisco 94143-0448.
Theis J
O'Farrell P H
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Article Info
Journal
Cell
Abbr.
Cell
ISSN
0092-8674
DOI
10.1016/0092-8674(88)90123-7
Published
1988-09-23
Pages
1081-90
Language
English
Region
United States
NLM ID
0413066
PMCID
PMC2753412
Subset
IM
Grants
NIGMS NIH HHS · R01 GM037193 · United States
NIGMS NIH HHS · R01 GM037193-10 · United States
External Links
PubMed source DOI: 10.1016/0092-8674(88)90123-7 PMC Full Text View journal details
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