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PMID: 3047166 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Characterization of a human hematopoietic progenitor cell capable of forming blast cell containing colonies in vitro.

The Journal of clinical investigation ·Vol. 82 ·No. 3 ·1988-09-00 ·Pages 1017-27

Brandt J, Baird N, Lu L, Srour E, Hoffman R

Abstract

A hematopoietic cell (CFU-B1) capable of producing blast cell containing colonies in vitro was detected using a semisolid culture system. The CFU-B1 has the capacity for self-renewal and commitment to a number of hematopoietic lineages. Monoclonal antibody to the human progenitor cell antigen-1 (HPCA-1) and a monoclonal antibody against the major histocompatibility class II antigen (HLA-DR) were used with fluorescence activated cell sorting to phenotype the CFU-B1. The CFU-B1 was found to express My10 but not HLA-DR antigen; experiments using complement-dependent cytotoxicity to eliminate DR positive cells confirmed this finding. Pretreatment of marrow cells with two chemotherapeutic agents, 5-fluorouracil and 4-hydroperoxycyclophosphamide facilitated detection of CFU-B1 derived colonies, while diminishing or totally inhibiting colony formation by other hematopoietic progenitor cells. CFU-B1-derived colony formation was dependent upon the addition of exogenous hematopoietic growth factors. Media conditioned either by the human bladder carcinoma cell line 5637 or lectin stimulated leukocytes, as well as recombinant granulocyte-macrophage colony stimulating factor, interleukin 3 or interleukin 1 alpha promoted blast cell colony formation. By contrast, neither recombinant erythropoietin, recombinant interleukin 4, purified macrophage colony stimulating factor or recombinant granulocyte colony-stimulating factor alone promoted blast cell colony formation.

MeSH Terms
Bone Marrow/physiology Bone Marrow Cells Cell Count Cell Differentiation/drug effects Cell Line Cell Separation Colony-Forming Units Assay Culture Media/pharmacology Growth Substances/pharmacology Hematopoiesis/drug effects Hematopoietic Cell Growth Factors Hematopoietic Stem Cells/classification,cytology,physiology Humans Interphase/drug effects Phenotype
Chemicals
Culture Media Growth Substances Hematopoietic Cell Growth Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brandt J
Division of Hematology/Oncology, Indiana University School of Medicine, Indianapolis 46223.
Baird N
Lu L
Srour E
Hoffman R
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113658
Published
1988-09-00
Pages
1017-27
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303616
Subset
IM
Grants
NCI NIH HHS · CA-34841 · United States
External Links
PubMed source DOI: 10.1172/JCI113658 PMC Full Text View journal details
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