Effects of release factor context at UAA codons in Escherichia coli.
In Escherichia coli, nonsense suppression at UAA codons is governed by the competition between a suppressor tRNA and the translational release factors RF1 and RF2. We have employed plasmids carrying the genes for RF1 and RF2 to measure release factor preference at UAA codons at 13 different sites in the lacI gene. We show here that the activity of RF1 and RF2 varies according to messenger context. RF1 is favored at UAA codons which are efficiently suppressed. RF2 is preferred at poorly suppressed sites.
12 references, click to expand
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Context effects: translation of UAG codon by suppressor tRNA is affected by the sequence following UAG in the message.J Mol Biol. 1983 Feb 15;164(1):73-87 PMID: 6188841
-
Nonsense suppression context effects in Escherichia coli bacteriophage T4.Mol Gen Genet. 1987 May;207(2-3):517-8 PMID: 3302602
-
The influence of the reading context upon the suppression of nonsense codons.Mol Gen Genet. 1977 Mar 7;151(2):137-49 PMID: 327262
-
Correlation of nonsense sites in the lacI gene with specific codons in the nucleotide sequence.Nature. 1978 Aug 24;274(5673):770-5 PMID: 355892
-
The influence of codon context on genetic code translation.Nature. 1980 Jul 10;286(5769):123-7 PMID: 7402305
-
Quantitative analysis of the relationship between nucleotide sequence and functional activity.Nucleic Acids Res. 1986 Aug 26;14(16):6661-79 PMID: 3092188
-
Mapping and complementation studies of the gene for release factor 1.J Bacteriol. 1986 Dec;168(3):1066-9 PMID: 3782033
-
A temperature-sensitive mutant of Escherichia coli that shows enhanced misreading of UAG/A and increased efficiency for some tRNA nonsense suppressors.Mol Gen Genet. 1984;193(1):38-45 PMID: 6419024
-
Genetic screen for cloned release factor genes.J Bacteriol. 1984 Apr;158(1):362-4 PMID: 6201475
-
Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs.J Bacteriol. 1984 Jun;158(3):849-59 PMID: 6327642
-
Influence of modification next to the anticodon in tRNA on codon context sensitivity of translational suppression and accuracy.J Bacteriol. 1986 Jun;166(3):1022-7 PMID: 3086285
-
Effects of surrounding sequence on the suppression of nonsense codons.J Mol Biol. 1983 Feb 15;164(1):59-71 PMID: 6188840
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