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PMID: 3057452 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A new member of the ras gene superfamily identified in a rat liver cell line.

Nucleic acids research ·Vol. 16 ·No. 21 ·1988-11-11 ·Pages 9979-93

Bucci C, Frunzio R, Chiariotti L, Brown AL, Rechler MM, Bruni CB

Abstract

A new member of the ras genes superfamily was isolated from a cDNA library derived from a rat liver cell line (BRL-3A). The predicted 201 amino acids ras-like protein shows 30-35% homology with other members of the ras and ras-related gene products so far described. Conserved features include the GTP-binding and hydrolysis domains and the carboxyl terminal cysteine residues. A protein of the expected size (Mr 23,000) was synthesized in an in vitro transcription-translation system. The BRL-ras gene is present in single copy in the rat genome and is ubiquitously expressed at high levels in all tissues and cell lines examined.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line Cloning, Molecular Genes, ras Liver Molecular Sequence Data Protein Conformation Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins p21(ras) Rats Sequence Homology, Nucleic Acid
Chemicals
Proto-Oncogene Proteins Proto-Oncogene Proteins p21(ras)
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bucci C
Dipartimento di Biologia e Patologia Cellulare e Molecolare, Università di Napoli, Italy.
Frunzio R
Chiariotti L
Brown A L
Rechler M M
Bruni C B
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.21.9979
Published
1988-11-11
Pages
9979-93
Language
English
Region
England
NLM ID
0411011
PMCID
PMC338831
Subset
IM
Databases
GENBANK
X12535
External Links
PubMed source DOI: 10.1093/nar/16.21.9979 PMC Full Text View journal details
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