70-kD heat shock-related protein is one of at least two distinct cytosolic factors stimulating protein import into mitochondria.
We have developed an in vitro system in which the posttranslational import of Put2 (delta-pyrroline-5-carboxylate dehydrogenase), into yeast mitochondria is dependent on the addition of yeast postribosomal supernatant (PRS). When mRNA for a nuclear-encoded yeast mitochondrial matrix protein, Put2, was translated in a wheat germ cell-free system, import into posttranslationally added yeast mitochondria was negligible. However, when a yeast PRS was added, significant import was observed. The import stimulating activity of the yeast PRS was shown to consist of at least two distinct factors. One of these is the recently purified 70-kD heat shock-related protein Ssalp/Ssa2p, two proteins that are 98% homologous. The other factor is an N-ethylmaleimide-sensitive protein(s). Both factors act synergistically.
24 references, click to expand
-
Import of proteins into mitochondria: precursor forms of the extramitochondrially made F1-ATPase subunits in yeast.Proc Natl Acad Sci U S A. 1979 Jan;76(1):343-7 PMID: 154672
-
70K heat shock related proteins stimulate protein translocation into microsomes.Nature. 1988 Apr 28;332(6167):805-10 PMID: 3282179
-
A neutral metallo endoprotease involved in the processing of an F1-ATPase subunit precursor in mitochondria.J Biol Chem. 1982 Mar 25;257(6):3177-82 PMID: 6460766
-
Membrane and cytosolic components affecting transport of the precursor for ornithine carbamyltransferase into mitochondria.J Biol Chem. 1983 Jun 10;258(11):6667-70 PMID: 6853495
-
Transport of ornithine carbamoyltransferase precursor into mitochondria. Stimulation by potassium ion, magnesium ion, and a reticulocyte cytosolic protein(s).J Biol Chem. 1983 Jun 10;258(11):6671-4 PMID: 6853496
-
Proline utilization in Saccharomyces cerevisiae: analysis of the cloned PUT2 gene.Mol Cell Biol. 1983 Oct;3(10):1846-56 PMID: 6358862
-
Cell-free translation of messenger RNA in a wheat germ system.Methods Enzymol. 1983;96:38-50 PMID: 6656637
-
Import of polypeptides into isolated yeast mitochondria.Methods Enzymol. 1983;97:329-36 PMID: 6361475
-
A purified precursor polypeptide requires a cytosolic protein fraction for import into mitochondria.EMBO J. 1984 Mar;3(3):651-7 PMID: 6232136
-
Mutations of the heat inducible 70 kilodalton genes of yeast confer temperature sensitive growth.Cell. 1984 Oct;38(3):841-9 PMID: 6386178
-
RNA required for import of precursor proteins into mitochondria.Science. 1984 Dec 14;226(4680):1319-22 PMID: 6209799
-
Primary structure of the nuclear PUT2 gene involved in the mitochondrial pathway for proline utilization in Saccharomyces cerevisiae.Mol Cell Biol. 1984 Dec;4(12):2837-42 PMID: 6098824
-
The precursor to ornithine carbamyl transferase is transported to mitochondria as a 5S complex containing an import factor.Biochem Biophys Res Commun. 1985 Aug 30;131(1):289-98 PMID: 3899114
-
Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis.J Cell Biol. 1986 May;102(5):1543-50 PMID: 3517001
-
Speculations on the functions of the major heat shock and glucose-regulated proteins.Cell. 1986 Sep 26;46(7):959-61 PMID: 2944601
-
Protein translocation across the yeast microsomal membrane is stimulated by a soluble factor.J Cell Biol. 1986 Dec;103(6 Pt 2):2629-36 PMID: 3025220
-
Protein import into chloroplasts requires a chloroplast ATPase.Proc Natl Acad Sci U S A. 1987 May;84(10):3288-92 PMID: 2953027
-
Distinct steps in the import of ADP/ATP carrier into mitochondria.J Biol Chem. 1987 Jun 5;262(16):7528-36 PMID: 3034898
-
Soluble factors stimulating secretory protein translocation in bacteria and yeast can substitute for each other.Proc Natl Acad Sci U S A. 1987 Jun;84(11):3723-7 PMID: 3295868
-
Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.Mol Cell Biol. 1987 Jul;7(7):2568-77 PMID: 3302682
-
The role of protein structure in the mitochondrial import pathway. Analysis of the soluble F1-ATPase beta-subunit precursor.J Biol Chem. 1987 Nov 15;262(32):15598-604 PMID: 2890630
-
The cytosolic factor required for import of precursors of mitochondrial proteins into mitochondria.J Biol Chem. 1988 Mar 5;263(7):3188-93 PMID: 2830273
-
A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides.Nature. 1988 Apr 28;332(6167):800-5 PMID: 3282178
-
Purification of a membrane-associated protein complex required for protein translocation across the endoplasmic reticulum.Proc Natl Acad Sci U S A. 1980 Dec;77(12):7112-6 PMID: 6938958
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
