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PMID: 3059351 Published · ppublish English Journal Article

Stable expression of the tax gene of type I human T-cell leukemia virus in human T cells activates specific cellular genes involved in growth.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 24 ·1988-12-00 ·Pages 9733-7

Wano Y, Feinberg M, Hosking JB, Bogerd H, Greene WC

Abstract

Stable expression of the 40-kDa transactivator protein (Tax) from the type I human T-cell leukemia virus (HTLV-I) in Jurkat T cells leads to the activation and sustained expression of certain cellular genes that are transiently induced during normal T-cell growth. Cellular genes induced by Tax include those encoding the alpha subunit of the high-affinity interleukin 2 receptor (Tac), interleukin 2, and granulocyte/macrophage colony-stimulating factor. Tax induction of the interleukin 2 gene is synergistically amplified by mitogens that augment cytoplasmic levels of calcium. These changes in the pattern of cellular gene expression reflect a specific action of Tax, as they are undetectable in isogenically matched control cell lines expressing antisense tax cDNA. The spectrum of cellular genes regulated by Tax appears to be restricted: several other T-cell genes, either inducibly or constitutively expressed, are unaffected by this viral protein. These cell lines constitutively expressing Tax provide valuable reagents to explore the molecular basis for Tax action and to delineate the full spectrum of cellular genes regulated by this retroviral gene product.

MeSH Terms
Animals Bacterial Proteins Blotting, Southern Colony-Stimulating Factors/genetics Deoxyribonucleases, Type II Site-Specific/metabolism Gene Expression Regulation Granulocyte-Macrophage Colony-Stimulating Factor Growth Substances/genetics HTLV-I Antigens/analysis Human T-lymphotropic virus 1/genetics Interleukin-2/genetics Plasmids Receptors, Interleukin-2/genetics T-Lymphocytes/analysis,ultrastructure Trans-Activators Transcription Factors/analysis
Chemicals
Bacterial Proteins Colony-Stimulating Factors Growth Substances HTLV-I Antigens Interleukin-2 Receptors, Interleukin-2 Trans-Activators Transcription Factors Granulocyte-Macrophage Colony-Stimulating Factor BglII endonuclease Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Wano Y
Howard Hughes Medical Institute, Duke University Medical Center, Durham, NC 27710.
Feinberg M
Hosking J B
Bogerd H
Greene W C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.24.9733
Published
1988-12-00
Pages
9733-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282853
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.24.9733 PMC Full Text View journal details
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