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PMID: 3067082 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Multicopy plasmid stability in Escherichia coli requires host-encoded functions that lead to plasmid site-specific recombination.

Molecular & general genetics : MGG ·Vol. 214 ·No. 1 ·1988-09-00 ·Pages 80-4

Stirling CJ, Stewart G, Sherratt DJ

Abstract

The heritable stability of the multicopy plasmid ColE1 and its natural relatives, requires the presence in the plasmid of a site (cer in ColE1) that acts as a substrate for site-specific recombination, thereby maintaining plasmids in the monomeric state. Multimerization, promoted by homologous recombination, leads to plasmid loss. Here we show that the Escherichia coli chromosome encodes at least two unlinked functions that act on cer and its analogous sites, to promote stabilizing site-specific recombination. One of these functions is encoded by a gene residing on a cosmid that also contains the argI and pyrB genes, mapping it to the 96-97 min region of the E. coli map.

MeSH Terms
Bacterial Proteins/genetics Colicins/genetics Cosmids Escherichia coli/genetics Genes, Bacterial Genetic Complementation Test Mutation Phenotype Plasmids Recombination, Genetic Restriction Mapping
Chemicals
Bacterial Proteins Colicins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stirling C J
Institute of Genetics, University of Glasgow, UK.
Stewart G
Sherratt D J
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21 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00340183
Published
1988-09-00
Pages
80-4
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00340183
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