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PMID: 3584064 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A site-specific recombination function in Staphylococcus aureus plasmids.

Journal of bacteriology ·Vol. 169 ·No. 6 ·1987-06-00 ·Pages 2601-10

Gennaro ML, Kornblum J, Novick RP

Abstract

All known small staphylococcal plasmids possess one or two recombination sites at which site-specific cointegrate formation occurs. One of these sites, RSA, is present on two small multicopy plasmids, pT181 and pE194; it consists of 24 base pairs of identity in the two plasmids, the "core," flanked by some 50 base pairs of decreasing homology. Here we show that recombination at RSA is recA independent and is mediated by a plasmid-encoded, trans-acting protein, Pre (plasmid recombination). Pre-mediated recombination is site specific in that it occurs within the core sequence of RSA in a recA1 host. Recombination also occurs between two intramolecular RSA sites. Unlike site-specific recombination systems encoded by other plasmids, Pre-RSA is not involved in plasmid maintenance.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Genes Genes, Bacterial Plasmids Promoter Regions, Genetic Recombination, Genetic Staphylococcus aureus/genetics
Chemicals
Bacterial Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gennaro M L
Kornblum J
Novick R P
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.6.2601-2610.1987
Published
1987-06-00
Pages
2601-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212133
Subset
IM
Grants
NIGMS NIH HHS · R01-GM 14372 · United States
Databases
GENBANK
J01764, J01765
External Links
PubMed source DOI: 10.1128/jb.169.6.2601-2610.1987 PMC Full Text View journal details
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