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PMID: 3083416 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Repetitive segmental structure of the transducin beta subunit: homology with the CDC4 gene and identification of related mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 7 ·1986-04-00 ·Pages 2162-6

Fong HK, Hurley JB, Hopkins RS, Miake-Lye R, Johnson MS, Doolittle RF, Simon MI

Abstract

Retinal transducin, a guanine nucleotide regulatory protein (referred to as a G protein) that activates a cGMP phosphodiesterase in photoreceptor cells, is comprised of three subunits. We have identified and analyzed cDNA clones of the bovine transducin beta subunit that may be highly conserved or identical to that in other G proteins. From the cDNA nucleotide sequence of the entire coding region, the primary structure of a 340-amino acid protein was deduced. The encoded beta subunit has a Mr of 37,375 and is comprised of repetitive homologous segments arranged in tandem. Furthermore, significant homology in primary structure and segmental sequence exists between the beta subunit and the yeast CDC4 gene product. The Mr 37,375 beta subunit polypeptide is encoded by a 2.9-kilobase (kb) mRNA. However, there exists in retina other beta-related mRNAs that are divergent from the 2.9-kb mRNA on the basis of oligonucleotide and primer-extended probe hybridizations. All mammalian tissues and clonal cell lines that have been examined contain at least two beta-related mRNAs, usually 1.8 and 2.9 kb in length. These results suggest that the mRNAs are the processed products of a small number of closely related genes or of a single highly complex beta gene.

MeSH Terms
Amino Acid Sequence Animals Cattle Cloning, Molecular DNA/genetics Fungal Proteins/genetics GTP-Binding Proteins/genetics Genes, Fungal Membrane Proteins/genetics Mice Saccharomyces cerevisiae/genetics Transducin
Chemicals
Fungal Proteins Membrane Proteins DNA GTP-Binding Proteins Transducin
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fong H K
Hurley J B
Hopkins R S
Miake-Lye R
Johnson M S
Doolittle R F
Simon M I
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.7.2162
Published
1986-04-00
Pages
2162-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323251
Subset
IM
Grants
NIGMS NIH HHS · GM34236 · United States
Databases
GENBANK
M13236
External Links
PubMed source DOI: 10.1073/pnas.83.7.2162 PMC Full Text View journal details
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