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PMID: 3084450 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Naturally occurring Staphylococcus epidermidis plasmid expressing constitutive macrolide-lincosamide-streptogramin B resistance contains a deleted attenuator.

Journal of bacteriology ·Vol. 166 ·No. 2 ·1986-05-00 ·Pages 479-83

Lampson BC, Parisi JT

Abstract

A naturally occurring constitutive macrolide-lincosamide-streptogramin B (MLS) resistance plasmid, pNE131, from Staphylococcus epidermidis was chosen to study the molecular basis of constitutive expression. Restriction and functional maps of pNE131 are presented along with the nucleotide sequence of ermM, the gene which mediates constitutive MLS resistance. Sharing 98% sequence homology within the 870-base-pair Sau3A-TaqI fragment, ermM appears to be almost identical to ermC, the inducible MLS resistance determinant from S. aureus (pE194). The two genes share nearly identical sequences, except in the 5' promoter region of ermM. Constitutive expression of ermM is due to the deletion of 107 base pairs relative to ermC; the deletion removes critical sequences for attenuation, resulting in constitutive methylase expression.

MeSH Terms
Amino Acid Sequence Anti-Bacterial Agents/pharmacology Base Sequence Chromosome Deletion DNA (Cytosine-5-)-Methyltransferases/metabolism Drug Resistance, Microbial Gene Expression Regulation Lincosamides Macrolides Nucleic Acid Conformation Phenotype Plasmids Staphylococcus epidermidis/drug effects,genetics Virginiamycin/pharmacology
Chemicals
Anti-Bacterial Agents Lincosamides Macrolides Virginiamycin DNA (Cytosine-5-)-Methyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lampson B C
Parisi J T
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33 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.166.2.479-483.1986
Published
1986-05-00
Pages
479-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC214629
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.166.2.479-483.1986 PMC Full Text View journal details
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