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PMID: 3092189 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins.

Nucleic acids research ·Vol. 14 ·No. 16 ·1986-08-26 ·Pages 6745-63

Gribskov M, Burgess RR

Abstract

We show, using dot matrix comparisons and statistical analysis of sequence alignments, that seven sequenced sigma factors, E. coli sigma-70 and sigma-32, B. subtilis sigma-43 and sigma-29, phage SP01 gene products 28 and 34, and phage T4 gene product 55, comprise a homologous family of proteins. Sigma-70, sigma-32, and sigma-43 each have two copies of a sequence similar to the helix-turn-helix DNA binding motif seen in CRP, and lambda repressor and cro proteins. B. subtilis sigma-29, SP01 gp28, and SP01 gp34 have at least one copy similar to this sequence. We propose that a second sequence, conserved in all seven proteins is the core RNA polymerase binding site. A third region, present only in sigma-70 and sigma-43, may also be involved in interaction with core. Available mutational evidence supports our model for sigma factor structure.

MeSH Terms
Amino Acid Sequence Bacillus subtilis/genetics Bacteriophages/genetics Binding Sites DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics Mutation Protein Binding Sigma Factor/genetics Species Specificity T-Phages/genetics Transcription Factors/genetics
Chemicals
Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gribskov M
Burgess R R
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53 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.16.6745
Published
1986-08-26
Pages
6745-63
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311678
Subset
IM
Grants
NCI NIH HHS · CA 07175 · United States
NCI NIH HHS · CA 09135 · United States
NIGMS NIH HHS · GM 28575 · United States
External Links
PubMed source DOI: 10.1093/nar/14.16.6745 PMC Full Text View journal details
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