Home LiteratureArticle Details
PMID: 3092737 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High-efficiency transformation of Streptococcus lactis protoplasts by plasmid DNA.

Applied and environmental microbiology ·Vol. 52 ·No. 2 ·1986-08-00 ·Pages 394-5

Simon D, Rouault A, Chopin MC

Abstract

Streptococcus lactis IL1403 protoplasts were transformed by plasmid pIL204 (5.5 kilobases), which conferred erythromycin resistance with an average efficiency of 5 X 10(6) transformants per microgram of supercoiled DNA. The procedure used and transformation efficiencies obtained were close to those described for Bacillus subtilis (G. Chang and S. N. Cohen, Mol. Gen. Genet. 168:111-115, 1979).

MeSH Terms
Drug Resistance, Microbial Erythromycin/pharmacology Kinetics Lactococcus lactis/drug effects,genetics,growth & development Protoplasts/physiology R Factors Transformation, Bacterial
Chemicals
Erythromycin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Simon D
Rouault A
Chopin M C
References (10)
10 references, click to expand
  1. Molecular cloning of the lactose-metabolizing genes from Streptococcus lactis.
    Appl Environ Microbiol. 1984 Aug;48(2):347-51 PMID: 6091547
  2. Improved medium for lactic streptococci and their bacteriophages.
    Appl Microbiol. 1975 Jun;29(6):807-13 PMID: 16350018
  3. Transformation of Streptococcus lactis Protoplasts by Plasmid DNA.
    Appl Environ Microbiol. 1982 May;43(5):1213-5 PMID: 16346019
  4. Two plasmid-determined restriction and modification systems in Streptococcus lactis.
    Plasmid. 1984 May;11(3):260-3 PMID: 6087394
  5. Transformation of Bacillus stearothermophilus with plasmid DNA and characterization of shuttle vector plasmids between Bacillus stearothermophilus and Bacillus subtilis.
    J Bacteriol. 1982 Mar;149(3):824-30 PMID: 6277855
  6. Effect of Ca2+ ions on plasmid transformation of Streptococcus lactis protoplasts.
    Appl Environ Microbiol. 1985 Oct;50(4):1100-2 PMID: 3936417
  7. Cloning in Streptococcus lactis of plasmid-mediated UV resistance and effect on prophage stability.
    Appl Environ Microbiol. 1986 Feb;51(2):233-7 PMID: 3006588
  8. High frequency transformation of Bacillus subtilis protoplasts by plasmid DNA.
    Mol Gen Genet. 1979 Jan 5;168(1):111-5 PMID: 107388
  9. Plasmid transformation of Streptococcus lactis protoplasts: optimization and use in molecular cloning.
    Appl Environ Microbiol. 1984 Aug;48(2):252-9 PMID: 6091544
  10. Cloning and expression of a Streptococcus cremoris proteinase in Bacillus subtilis and Streptococcus lactis.
    Appl Environ Microbiol. 1985 Jul;50(1):94-101 PMID: 2992377
Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
0099-2240
DOI
10.1128/aem.52.2.394-395.1986
Published
1986-08-00
Pages
394-5
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC203539
Subset
IM
External Links
PubMed source DOI: 10.1128/aem.52.2.394-395.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2