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PMID: 3097027 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Calcium ionophore A23187 as a regulator of gene expression in mammalian cells.

The Journal of cell biology ·Vol. 103 ·No. 6 Pt 1 ·1986-12-00 ·Pages 2145-52

Resendez E, Ting J, Kim KS, Wooden SK, Lee AS

Abstract

The calcium ionophore A23187 can reversibly induce the expression of two glucose-regulated genes, p3C5 and p4A3. This induction requires a continuous presence of the ionophore for over 2 h. Although extracellular Ca2+ is important for the optimal effect of A23187, it is not necessary for the induction, since a similar response with a lower magnitude can be triggered in cells cultured in low Ca2+ medium buffered with EGTA. Both the basal and induced levels of p3C5 and p4A3 transcripts can be modulated by the calmodulin antagonist W-7, indicating the involvement of Ca2+/calmodulin-associated pathways. In addition, the sensitivity of the A23187 induction to cycloheximide suggests that the induction process is dependent on de novo protein synthesis.

MeSH Terms
Animals Calcimycin/pharmacology Calcium/pharmacology Cell Line Cricetinae Genes/drug effects Genes, Regulator/drug effects HSP70 Heat-Shock Proteins Membrane Proteins/biosynthesis,genetics Nucleic Acid Hybridization Plasmids Transcription, Genetic/drug effects
Chemicals
HSP70 Heat-Shock Proteins Membrane Proteins glucose-regulated proteins Calcimycin Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Resendez E
Ting J
Kim K S
Wooden S K
Lee A S
References (33)
33 references, click to expand
  1. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters.
    J Biol Chem. 1982 Jul 10;257(13):7847-51 PMID: 7085651
  2. Highly conserved glucose-regulated protein in hamster and chicken cells: preliminary characterization of its cDNA clone.
    Proc Natl Acad Sci U S A. 1981 Aug;78(8):4922-5 PMID: 6946438
  3. Regulation of membrane assembly during development of sarcoplasmic reticulum: the possible role of calcium.
    Ann N Y Acad Sci. 1982;402:485-514 PMID: 6820247
  4. Biochemical characterization of the mammalian stress proteins and identification of two stress proteins as glucose- and Ca2+-ionophore-regulated proteins.
    J Biol Chem. 1983 Jun 10;258(11):7102-11 PMID: 6406494
  5. ADP-ribosylation of the Mr 83,000 stress-inducible and glucose-regulated protein in avian and mammalian cells: modulation by heat shock and glucose starvation.
    Proc Natl Acad Sci U S A. 1983 Aug;80(15):4664-8 PMID: 6576354
  6. Phorbol 12-myristate 13-acetate activates rabbit neutrophils without an apparent rise in the level of intracellular free calcium.
    Biochem Biophys Res Commun. 1983 Jul 29;114(2):638-45 PMID: 6411090
  7. Expression of human alpha-tubulin genes: interspecies conservation of 3' untranslated regions.
    Mol Cell Biol. 1983 Oct;3(10):1738-45 PMID: 6646120
  8. Induction of two genes by glucose starvation in hamster fibroblasts.
    Proc Natl Acad Sci U S A. 1984 Feb;81(4):988-92 PMID: 6583707
  9. Biochemical characterization of the 94- and 78-kilodalton glucose-regulated proteins in hamster fibroblasts.
    J Biol Chem. 1984 Apr 10;259(7):4616-21 PMID: 6707023
  10. Calcium messenger system: an integrated view.
    Physiol Rev. 1984 Jul;64(3):938-84 PMID: 6330778
  11. Use of a cell cycle mutant to delineate the critical period for the control of histone mRNA levels in the mammalian cell cycle.
    Mol Cell Biol. 1984 Nov;4(11):2364-9 PMID: 6513920
  12. Calcium ionophore A23187 induces expression of glucose-regulated genes and their heterologous fusion genes.
    Mol Cell Biol. 1985 Jun;5(6):1212-9 PMID: 3929069
  13. Inhibition of the heat shock response and synthesis of glucose-regulated proteins in Ca2+-deprived rat hepatoma cells.
    Biochem Biophys Res Commun. 1985 Sep 16;131(2):868-76 PMID: 3931641
  14. Uncoating ATPase is a member of the 70 kilodalton family of stress proteins.
    Cell. 1986 Apr 11;45(1):3-13 PMID: 2937542
  15. An Hsp70-like protein in the ER: identity with the 78 kd glucose-regulated protein and immunoglobulin heavy chain binding protein.
    Cell. 1986 Jul 18;46(2):291-300 PMID: 3087629
  16. A calcium ionophore-inducible cellular promoter is highly active and has enhancerlike properties.
    Mol Cell Biol. 1986 Apr;6(4):1235-43 PMID: 3023878
  17. Large changes in intracellular pH and calcium observed during heat shock are not responsible for the induction of heat shock proteins in Drosophila melanogaster.
    Mol Cell Biol. 1986 May;6(5):1767-75 PMID: 3097504
  18. The apparent binding constant of glycoletherdiaminetetraacetic acid for calcium at neutral pH.
    J Biochem. 1968 Aug;64(2):255-7 PMID: 5715508
  19. Isolation of temperature sensitive mammalian cells by selective detachment.
    J Cell Physiol. 1973 Dec;82(3):325-31 PMID: 4359531
  20. Control of cell division in yeast using the ionophore, A23187 with calcium and magnesium.
    Nature. 1974 Oct 18;251(5476):626-7 PMID: 4423924
  21. The role of calcium ions in initiating transformation of lymphocytes.
    Nature. 1974 Sep 27;251(5473):324-7 PMID: 4427658
  22. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  23. Permeability of a cell junction and the local cytoplasmic free ionized calcium concentration: a study with aequorin.
    J Membr Biol. 1976 AUG 27;28(1):87-119 PMID: 787527
  24. The effect of intracellular calcium ions on adrenaline-stimulated adenosine 3':5'-cyclic monophosphate concentrations in pigeon erythrocytes, studied by using the ionophore A23187.
    Biochem J. 1976 Aug 15;158(2):211-21 PMID: 186033
  25. On the role of calcium as second messenger in liver for the hormonally induced activation of glycogen phosphorylase.
    Biochim Biophys Acta. 1977 Feb 28;496(2):448-57 PMID: 189844
  26. Calcium dependence of glucocorticoid-induced lymphocytolysis.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):638-42 PMID: 322136
  27. Ionophore-mediated calcium influx effects on the post-synaptic muscle fibre membrane.
    Nature. 1977 Dec 1;270(5636):441-3 PMID: 339103
  28. Inhibition by calcium ions of adenosine cyclic monophosphate formation in sealed pigeon erythrocyte 'ghosts'. A study using the photoprotein obelin.
    Biochem J. 1978 Oct 15;176(1):53-66 PMID: 215135
  29. In cultured chick embryo fibroblasts the hexose transport components are not the 75 000 and 95 000 dalton polypeptides synthesized following glucose deprivation.
    Can J Biochem. 1980 Oct;58(10):1179-88 PMID: 7459679
  30. The accumulation of three specific proteins related to glucose-regulated proteins in a temperature-sensitive hamster mutant cell line K12.
    J Cell Physiol. 1981 Jan;106(1):119-25 PMID: 7204505
  31. Selective stimulation of the synthesis of an 80,000-dalton protein by calcium ionophores.
    J Biol Chem. 1981 Jun 10;256(11):5309-12 PMID: 6165714
  32. N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide, a calmodulin antagonist, inhibits cell proliferation.
    Proc Natl Acad Sci U S A. 1981 Jul;78(7):4354-7 PMID: 6945588
  33. Transcriptional regulation of two genes specifically induced by glucose starvation in a hamster mutant fibroblast cell line.
    J Biol Chem. 1983 Jan 10;258(1):597-603 PMID: 6294115
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.103.6.2145
Published
1986-12-00
Pages
2145-52
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2114569
Subset
IM
Grants
NCI NIH HHS · CA-27607 · United States
NIGMS NIH HHS · GM-9805-MRC · United States
External Links
PubMed source DOI: 10.1083/jcb.103.6.2145 PMC Full Text View journal details
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