Home LiteratureArticle Details
PMID: 3098559 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Expression of a family of psbA genes encoding a photosystem II polypeptide in the cyanobacterium Anacystis nidulans R2.

The EMBO journal ·Vol. 5 ·No. 11 ·1986-11-00 ·Pages 2789-98

Golden SS, Brusslan J, Haselkorn R

Abstract

The genome of the cyanobacterium Anacystis nidulans R2 contains three genes (psbA) for the QB protein of photosystem II. This protein is essential for oxygenic photosynthetic electron tansport, and is the target for several herbicides which act by binding directly to the photosynthetic apparatus. Transcripts from the three Anacystis psbA genes are present in wild-type cells at different steady-state levels. The nucleotide sequences of two of the genes, psbAII and psbAIII, predict a protein having the same amino acid sequence which differs from that of the psbAI gene by 25 (out of 360) residues. Inactivation of each of the psbA genes in the Anacystis chromosome, singly or in pairs, shows that each of the genes is capable of producing sufficient functional QB protein to support normal photoautotrophic growth.

MeSH Terms
Amino Acid Sequence Base Sequence Chlorophyll/genetics Cyanobacteria/genetics,metabolism Genes Light-Harvesting Protein Complexes Nucleic Acid Conformation Photosynthesis Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins/genetics Transcription, Genetic
Chemicals
Light-Harvesting Protein Complexes Photosynthetic Reaction Center Complex Proteins Photosystem II Protein Complex Plant Proteins Chlorophyll
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Golden S S
Brusslan J
Haselkorn R
References (27)
27 references, click to expand
  1. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  2. Photoaffinity labeling of an herbicide receptor protein in chloroplast membranes.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):981-5 PMID: 16592984
  3. New versatile plasmid vectors for expression of hybrid proteins coded by a cloned gene fused to lacZ gene sequences encoding an enzymatically active carboxy-terminal portion of beta-galactosidase.
    Gene. 1983 Nov;25(1):71-82 PMID: 6319233
  4. Two alkaline phosphatase genes positioned in tandem in Bacillus licheniformis MC14 require different RNA polymerase holoenzymes for transcription.
    Proc Natl Acad Sci U S A. 1985 Feb;82(4):1035-9 PMID: 3856244
  5. Molecular Basis of Herbicide Resistance in Amaranthus hybridus.
    Science. 1983 Dec 23;222(4630):1346-9 PMID: 17773338
  6. Regulation of the synthesis of ribosomes and ribosomal components.
    Annu Rev Biochem. 1984;53:75-117 PMID: 6206783
  7. Differences in mRNA levels in Anabaena living freely or in symbiotic association with Azolla.
    EMBO J. 1986 Jan;5(1):29-35 PMID: 2869943
  8. In vitro packaging of lambda and cosmid DNA.
    Methods Enzymol. 1979;68:299-309 PMID: 161604
  9. Escherichia coli promoter sequences predict in vitro RNA polymerase selectivity.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):789-800 PMID: 6364042
  10. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325.
    Gene. 1980 May;9(3-4):287-305 PMID: 6248430
  11. Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene.
    J Bacteriol. 1983 Jan;153(1):562-5 PMID: 6336742
  12. Nucleotide sequence of the gene for the M(r) 32,000 thylakoid membrane protein from Spinacia oleracea and Nicotiana debneyi predicts a totally conserved primary translation product of M(r) 38,950.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7699-703 PMID: 16593262
  13. Nucleotide sequence of a multiple-copy gene for the B protein of photosystem II of a cyanobacterium.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2693-7 PMID: 16578775
  14. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  15. Mutation to herbicide resistance maps within the psbA gene of Anacystis nidulans R2.
    Science. 1985 Sep 13;229(4718):1104-7 PMID: 3929379
  16. Gene expression during development of Myxococcus xanthus. Analysis of the genes for protein S.
    J Mol Biol. 1984 Jun 5;175(4):469-92 PMID: 6204058
  17. Optimal conditions for genetic transformation of the cyanobacterium Anacystis nidulans R2.
    J Bacteriol. 1984 Apr;158(1):36-42 PMID: 6425267
  18. Hybridization of denatured RNA transferred or dotted nitrocellulose paper.
    Methods Enzymol. 1983;100:255-66 PMID: 6194404
  19. In vitro insertional mutagenesis with a selectable DNA fragment.
    Gene. 1984 Sep;29(3):303-13 PMID: 6237955
  20. Chlamydomonas reinhardii gene for the 32 000 mol. wt. protein of photosystem II contains four large introns and is located entirely within the chloroplast inverted repeat.
    EMBO J. 1984 Dec 1;3(12):2753-62 PMID: 16453578
  21. The rapidly metabolized 32,000-dalton polypeptide of the chloroplast is the "proteinaceous shield" regulating photosystem II electron transport and mediating diuron herbicide sensitivity.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1572-6 PMID: 6940173
  22. Chloroplast-coded atrazine resistance in Solanum nigrum: psbA loci from susceptible and resistant biotypes are isogenic except for a single codon change.
    Nucleic Acids Res. 1984 Dec 21;12(24):9489-96 PMID: 6514581
  23. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  24. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
  25. Control of macromolecular composition and cell division in the blue-green algae Anacystis nidulans.
    J Gen Microbiol. 1974 Aug;83(2):399-405 PMID: 4214895
  26. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  27. Metronidazole and the isolation of temperature-sensitive photosynthetic mutants in cyanobacteria.
    J Bioenerg Biomembr. 1980 Aug;12(3-4):277-95 PMID: 6783642
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-11-00
Pages
2789-98
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167224
Subset
IM
Databases
GENBANK
X04616, X04617, X04618
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2