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PMID: 309940 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

T-tubule swelling in hypertonic solutions: a freeze substitution study.

The Journal of physiology ·Vol. 283 ·1978-10-00 ·Pages 133-40

Franzini-Armstrong C, Heuser JE, Reese TS, Somlyo AP, Somlyo AV

Abstract

Striated muscles from Rana pipiens have been exposed for variable periods of time to Ringer solutions made hypertonic by addition of either sucrose or sodium chloride. The muscles have been rapid-frozen and then prepared for electron microscopy by either freeze-substitution, freeze-fracture or cryoultramicrotomy. The only compartment greatly affected by hypertonicity is the transverse tubular system, which is visibly swollen. None of the elements of the sarcoplasmic reticulum increase in size.

MeSH Terms
Animals Anura Freeze Fracturing Hypertonic Solutions In Vitro Techniques Microtubules/physiology,ultrastructure Muscles/physiology,ultrastructure Rana pipiens Sarcoplasmic Reticulum/physiology
Chemicals
Hypertonic Solutions
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Franzini-Armstrong C
Heuser J E
Reese T S
Somlyo A P
Somlyo A V
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34 references, click to expand
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Article Info
Journal
The Journal of physiology
Abbr.
J Physiol
ISSN
0022-3751
DOI
10.1113/jphysiol.1978.sp012492
Published
1978-10-00
Pages
133-40
Language
English
Region
England
NLM ID
0266262
PMCID
PMC1282769
Subset
IM
External Links
PubMed source DOI: 10.1113/jphysiol.1978.sp012492 PMC Full Text View journal details
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