Specific and sensitive plate assay for bacterial lipases.

Applied and environmental microbiology ·Vol. 53 ·No. 1 ·1987-01-00 ·Pages 211-3

Kouker G, Jaeger KE

Abstract

A plate assay to detect bacterial lipase (EC 3.1.1.3) in a medium containing trioleoylglycerol and the fluorescent dye rhodamine B is presented. Substrate hydrolysis causes the formation of orange fluorescent halos around bacterial colonies visible upon UV irradiation. The logarithm of lipase activity from cell-free culture supernatants is linearly correlated with the diameter of halos, thereby allowing quantitation of lipase activities ranging from 1 to 30 nkat.

MeSH Terms

Bacillus subtilis/enzymology Bacteria/enzymology Escherichia coli/enzymology Lipase/analysis Olive Oil Plant Oils Pseudomonas aeruginosa/enzymology Rhodamines Serratia marcescens/enzymology Staphylococcus aureus/enzymology

Chemicals

Olive Oil Plant Oils Rhodamines Lipase rhodamine B

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kouker G

Jaeger K E

References (10)

10 references, click to expand

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Detection and determination of lipase (acylglycerol hydrolase) activity from various sources.
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Ultrathin-layer agar gels: a novel print technique for ultrathin-layer isoelectric focusing of enzymes.
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Mikrobiologiia. 1975 Mar-Apr;44(2):365-6 PMID: 1226152
A simple method for the detection of lipolytic activity of micro-organisms and some observations on the influence of the contact between cells and fatty substrates.
Antonie Van Leeuwenhoek. 1957;23(1):15-22 PMID: 13425509
A new assay of microbial lipases with emulsified trioleoyl glycerol.
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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.53.1.211-213.1987

Published

1987-01-00

Pages

211-3

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC203632

Subset

External Links

PubMed source DOI: 10.1128/aem.53.1.211-213.1987 PMC Full Text View journal details

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