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PMID: 6633171 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Detection and determination of lipase (acylglycerol hydrolase) activity from various sources.

Lipids ·Vol. 18 ·No. 9 ·1983-09-00 ·Pages 650-7

Jensen RG

Abstract

Methods for the detection and determination of lipases (acylglycerol hydrolases) and preparation of assays are reviewed including substrates, conditions and screening. Some newer methods for the determination of lipase activity are discussed. Several of these are: (a) titrimetry, (b) colorimetry of Cu soaps of free fatty acids (FFA), (c) colorimetry of chromophores in the acyl chain of FFA or in glycerol, (d) radioassay, (e) gas liquid chromatography, (f) enzymatic treatment of FFA and measurement of the resulting products, and (g) direct immunological determination of the lipase. Examples and sensitivities are given and advantages and disadvantages are described.

MeSH Terms
Chromatography, Gas/methods Copper Fatty Acids/analysis Hydrogen-Ion Concentration Lipase/analysis,immunology Spectrophotometry/methods Substrate Specificity
Chemicals
Fatty Acids Copper Lipase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Jensen R G
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50 references, click to expand
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Article Info
Journal
Lipids
Abbr.
Lipids
ISSN
0024-4201
DOI
10.1007/BF02534677
Published
1983-09-00
Pages
650-7
Language
English
Region
United States
NLM ID
0060450
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02534677 View journal details
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