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PMID: 3106320 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Functional expression of plastid allophycocyanin genes in a cyanobacterium.

Journal of bacteriology ·Vol. 169 ·No. 5 ·1987-05-00 ·Pages 1830-5

de Lorimier R, Guglielmi G, Bryant DA, Stevens SE

Abstract

In Cyanophora paradoxa, the allophycocyanin apoprotein subunits, alpha and beta, are encoded in the cyanelle (plastid) genome. These genes were transferred to the cyanobacterium Synechococcus sp. PCC 7002 on a plasmid replicon. Phycobilisomes isolated from transformed cyanobacteria were found to contain C. paradoxa allophycocyanin subunits. Thus, these plastid genes are expressed in the cyanobacterium as polypeptides which become linked to a chromophore and are incorporated into the light-harvesting apparatus.

MeSH Terms

Cloning, Molecular Cyanobacteria/genetics Dinoflagellida/genetics Gene Expression Regulation Genes Isoelectric Point Light-Harvesting Protein Complexes Molecular Weight Phycobilisomes Phycocyanin/genetics Pigments, Biological/genetics Plant Proteins/physiology Plasmids

Chemicals

Light-Harvesting Protein Complexes Phycobilisomes Pigments, Biological Plant Proteins allophycocyanin Phycocyanin

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

de Lorimier R

Guglielmi G

Bryant D A

Stevens S E

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.169.5.1830-1835.1987

Published

1987-05-00

Pages

1830-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC212032

Subset

Grants

NIGMS NIH HHS · GM 31625 · United States

External Links

PubMed source DOI: 10.1128/jb.169.5.1830-1835.1987 PMC Full Text View journal details

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Functional expression of plastid allophycocyanin genes in a cyanobacterium.

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