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PMID: 3118332 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Gene cluster for streptomycin biosynthesis in Streptomyces griseus: nucleotide sequence of three genes and analysis of transcriptional activity.

Nucleic acids research ·Vol. 15 ·No. 19 ·1987-10-12 ·Pages 8041-56

Distler J, Ebert A, Mansouri K, Pissowotzki K, Stockmann M, Piepersberg W

Abstract

Three streptomycin (SM) production genes from Streptomyces griseus clustered around aphD, the major resistance gene, have been sequenced: strB, coding for an aminocyclitol amidinotransferase, ORF5 (strR), a putative regulatory gene, and ORF1 (strD), possibly coding for a hexose nucleotidylating enzyme. Three promoters and at least five, partially overlapping, transcripts have been identified by S1 mapping and Northern blot experiments. aphD, the resistance gene, is transcribed from two promoters. One of them, located inside the strR gene, seems to be constitutive and the other is switched on later in the growth phase. The late transcripts cover the resistance gene (aphD) and a regulatory gene (strR) which controls the expression of strB.

MeSH Terms
Amino Acid Sequence Base Sequence Escherichia coli/genetics Genes Genes, Fungal Genes, Regulator Molecular Sequence Data Sequence Homology, Nucleic Acid Streptomyces griseus/genetics Streptomycin/genetics Transcription, Genetic
Chemicals
Streptomycin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Distler J
Institut für Organische Chemie und Biochemie der Technischen Hochschule Darmstadt, FRG.
Ebert A
Mansouri K
Pissowotzki K
Stockmann M
Piepersberg W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.19.8041
Published
1987-10-12
Pages
8041-56
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306325
Subset
IM
Databases
GENBANK
Y00459
External Links
PubMed source DOI: 10.1093/nar/15.19.8041 PMC Full Text View journal details
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