Home LiteratureArticle Details
PMID: 3122211 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human immunoglobulin C lambda 6 gene encodes the Kern+Oz-lambda chain and C lambda 4 and C lambda 5 are pseudogenes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 9074-8

Dariavach P, Lefranc G, Lefranc MP

Abstract

Six nonallelic immunoglobulin lambda constant region genes have been previously characterized on a 40-kilobase stretch of DNA. The nucleotide sequences of the three upstream genes of this cluster (C lambda 1, C lambda 2, C lambda 3) have been determined by other workers and shown to encode, respectively, the isotypic Mcg, Kern-Oz-, and Kern-Oz+ constant region of the lambda chains. In this paper, we report the sequence of the three downstream genes of this cluster and show that two of them (C lambda 4 and C lambda 5) are pseudogenes. However, C lambda 6 encodes a Kern+Oz- chain and corresponds to the fourth isotype described among the lambda proteins sequenced so far. A potentially active J lambda (joining) segment, with the canonical heptamer and nonamer sequences for rearrangement, is located 1.5 kilobases upstream of C lambda 6. The amino acid sequence encoded by the C lambda 6 gene is compared with the constant region sequences of various monoclonal Bence Jones lambda proteins. Allotypic and isotypic differences confirm the polymorphism and complexity of the human C lambda locus.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Genes, Immunoglobulin Humans Immunoglobulin Allotypes/genetics Immunoglobulin Constant Regions/genetics Immunoglobulin lambda-Chains/genetics Molecular Sequence Data Multigene Family Pseudogenes Regulatory Sequences, Nucleic Acid
Chemicals
Immunoglobulin Allotypes Immunoglobulin Constant Regions Immunoglobulin lambda-Chains
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dariavach P
Laboratoire d'Immunogénétique, Université des Sciences et Techniques du Languedoc, Montpellier, France.
Lefranc G
Lefranc M P
References (53)
53 references, click to expand
  1. [The primary structure of a monoclonal IgA-immunoglobulin (IgA Tro), I: The amino acid sequence of the L-chain of lambda-type, subgroup II (author's transl)].
    Hoppe Seylers Z Physiol Chem. 1975 Aug;356(8):1333-5 PMID: 809330
  2. A new subgroup of human L-chains of the lambda-type. Primary structure of Bence-Jones protein DEL.
    Eur J Biochem. 1974 Dec 16;50(1):49-69 PMID: 4452363
  3. A new lambda-chain gene.
    Immunochemistry. 1975 Aug;12(8):643-52 PMID: 812801
  4. The variability of human lambda-chain constant regions and some relationships to V-regions sequences.
    Immunochemistry. 1976 Feb;13(2):149-55 PMID: 816731
  5. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  6. Human lambda-chain sequence variations and serologic associations.
    Immunochemistry. 1977 Jun;14(6):429-33 PMID: 407150
  7. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  8. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  9. Amino acid sequence of the variable region of the light (lambda) chain from human myeloma cryoimmunoglobulin IgG Hil.
    Biochemistry. 1978 May 2;17(9):1718-23 PMID: 418804
  10. Sequences at the somatic recombination sites of immunoglobulin light-chain genes.
    Nature. 1979 Jul 26;280(5720):288-94 PMID: 111144
  11. Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.
    Proc Natl Acad Sci U S A. 1979 Jul;76(7):3450-4 PMID: 115000
  12. An immunoglobulin heavy chain variable region gene is generated from three segments of DNA: VH, D and JH.
    Cell. 1980 Apr;19(4):981-92 PMID: 6769593
  13. Amino acid sequence of the lambda type light chain of a human IgGl myeloma protein (MOT) with unusual antigenicity: a possible new subgroup of lambda chain having a unique N-terminal sequence.
    Mol Immunol. 1980 Nov;17(11):1407-14 PMID: 6780787
  14. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  15. Assignment of the genes for human lambda immunoglobulin chains to chromosome 22.
    Nature. 1981 Nov 12;294(5837):173-5 PMID: 6795508
  16. Clustered arrangement of immunoglobulin lambda constant region genes in man.
    Nature. 1981 Dec 10;294(5841):536-40 PMID: 6273747
  17. Chromosomal location of human kappa and lambda immunoglobulin light chain constant region genes.
    J Exp Med. 1982 May 1;155(5):1480-90 PMID: 6802926
  18. Correlation between immunoglobulin light chain expression and variant translocation in Burkitt's lymphoma.
    Nature. 1982 Jul 29;298(5873):474-6 PMID: 6806672
  19. Primary structure of a human lambda-chain (Weir) of the Mcg type.
    Mol Immunol. 1982 Jul;19(7):901-6 PMID: 6811861
  20. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  21. Lambda Ig constant region genes are translocated to chromosome 8 in Burkitt's lymphoma with t(8;22).
    Nucleic Acids Res. 1983 Feb 25;11(4):1133-42 PMID: 6402758
  22. Variable amplification of immunoglobulin lambda light-chain genes in human populations.
    Nature. 1983 Jul 14-20;304(5922):172-4 PMID: 6306474
  23. An improved strategy for rapid direct sequencing of both strands of long DNA molecules cloned in a plasmid.
    Nucleic Acids Res. 1983 Aug 25;11(16):5521-40 PMID: 6310503
  24. Primary structure of the variable region of a human lambda VI light chain: Bence Jones protein SUT.
    J Immunol. 1983 Nov;131(5):2490-3 PMID: 6415173
  25. A new immunoglobulin marker.
    Biochem Biophys Res Commun. 1983 Dec 16;117(2):587-92 PMID: 6419741
  26. The breakpoint of the Philadelphia chromosome 22 in chronic myeloid leukaemia is distal to the immunoglobulin lambda light chain constant region genes.
    Mol Biol Med. 1983 Jul;1(1):11-9 PMID: 6436636
  27. A new selective phage cloning vector, lambda 2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI.
    Gene. 1984 Dec;32(1-2):217-24 PMID: 6099311
  28. Cloning and sequence analysis of an Ig lambda light chain mRNA expressed in the Burkitt's lymphoma cell line EB4.
    Nucleic Acids Res. 1985 Apr 25;13(8):2931-41 PMID: 3923440
  29. Human lambda light-chain constant region gene CMor lambda: the primary structure of lambda VI Bence Jones protein Mor.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3415-9 PMID: 3923477
  30. Cloning and sequencing of a rearranged V lambda gene from a Burkitt's lymphoma cell line expressing kappa light chains.
    Nucleic Acids Res. 1985 Jul 11;13(13):4921-34 PMID: 3927261
  31. Association of human lambda light chain V/J/C segments: serologic analysis and primary structure of the lambda VI Bence Jones protein THO.
    J Immunol. 1986 Jan;136(2):716-9 PMID: 3079791
  32. Identification of three new Ig lambda-like genes in man.
    J Exp Med. 1986 Feb 1;163(2):425-35 PMID: 3003227
  33. Human lambda light chain locus: organization and DNA sequences of three genomic J regions.
    Immunogenetics. 1987;25(1):63-70 PMID: 3102359
  34. 2 types of lambda polypeptide chains in human immunoglobulins.
    Science. 1967 May 19;156(3777):947-8 PMID: 4164622
  35. Peptide differences in purified mouse antibodies.
    Immunochemistry. 1967 Jan;4(1):31-6 PMID: 4166160
  36. Two types of lambda polypeptide chains in human immunoglobulins based on an amino acid substitution at position 190.
    Proc Natl Acad Sci U S A. 1967 May;57(5):1449-54 PMID: 4166768
  37. Linked groups of residues in immunoglobulin k chains.
    Nature. 1967 Oct 28;216(5113):330-2 PMID: 4168589
  38. Nonallelic behavior of the Oz groups in human lambda immunoglobulin chains.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):982-5 PMID: 5243936
  39. [Complete aminco acid sequence of Bence Jones portein Kern. A new subgroup of the immunoglobulin L-chains of lambda-type].
    Hoppe Seylers Z Physiol Chem. 1968 Jun;349(6):867-71 PMID: 4177553
  40. [The complete amino acid sequence of Bence Jones protein New (lambda-type). Subgroups in the variable part of immunoglobulin L-chains of the lambda-type].
    Hoppe Seylers Z Physiol Chem. 1968 Jul;349(7):945-51 PMID: 4177823
  41. Immunoglobulin lambda-chains. The complete amino acid sequence of a Bence-Jones protein.
    Biochem J. 1968 Dec;110(4):631-52 PMID: 4883841
  42. The amino acid sequence of a lambda type Bence-Jones protein. 3. The complete amino acid sequence and the location of the disulfide bridges.
    J Biol Chem. 1970 Apr 25;245(8):2171-6 PMID: 4909564
  43. [Primary structure of a monoclonal lambda-type immunoglobulin L-chain of subgroup IV (Bence Jones protein Bau): subgroups within subgroups].
    Hoppe Seylers Z Physiol Chem. 1970 Jun;351(6):763-7 PMID: 4193320
  44. Amino acid sequence of human lambda chains. II. Chymotryptic peptides and sequence of protein Ha.
    J Biol Chem. 1970 Sep 10;245(17):4475-87 PMID: 5532227
  45. Amino acid sequence of human lambda chains. 3. Tryptic peptides, chymotryptic peptides, and sequence of protein Bo.
    J Biol Chem. 1970 Sep 10;245(17):4488-507 PMID: 5532228
  46. [Structural rule of antibodies. Primary structure of a monoclonal immunoglobulin-L-chain of the lambda type, subgroup IV (Bence-Jones-protein Kern). V. The complete amino acid sequence and its genetic interpretation].
    Hoppe Seylers Z Physiol Chem. 1971 Feb;352(2):247-66 PMID: 5549568
  47. Isotypes in human immunoglobulin lambda-chains.
    Nat New Biol. 1971 Nov 10;234(45):58-61 PMID: 4108519
  48. Amino acid sequence of lambda chain of human immunoglobulin A.
    Immunochemistry. 1972 Feb;9(2):207-10 PMID: 5021231
  49. The primary structure of a monoclonal human lambda-type immunoglobulin L-chain of subgroup II (Bence-Jones protein NEI).
    Eur J Biochem. 1972 Mar 15;26(1):10-32 PMID: 5043326
  50. Amino acid sequence of the (lambda) light chain of a human myeloma immunoglobulin (IgG New).
    Biochemistry. 1974 Mar 12;13(6):1295-302 PMID: 4814727
  51. Primary structure of the Mcg lambda chain.
    Biochemistry. 1974 Sep 24;13(20):4102-14 PMID: 4415202
  52. [The primary structure of a monoclonal immunoglobulin L-chain of lambda-type, subgroup I (Bence-Jones protein Vor.) (author's transl)].
    Hoppe Seylers Z Physiol Chem. 1974 Jan;355(1):85-8 PMID: 4435715
  53. Primary structure of a deleted human lambda type immunoglobulin light chain containing carbohydrate: protein Sm lambda.
    Proc Natl Acad Sci U S A. 1975 Nov;72(11):4559-63 PMID: 812098
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.9074
Published
1987-12-00
Pages
9074-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299694
Subset
IM
Databases
GENBANK
J03009, J03010, J03011, M18337, M18338
External Links
PubMed source DOI: 10.1073/pnas.84.24.9074 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2