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PMID: 3124113 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Induction of fibronectin gene transcription and mRNA is a primary response to growth-factor stimulation of AKR-2B cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 4 ·1988-02-00 ·Pages 1119-23

Blatti SP, Foster DN, Ranganathan G, Moses HL, Getz MJ

Abstract

A cDNA library, prepared from poly(A)+ RNA isolated from quiescent AKR-2B cells 4 hr after stimulation with epidermal growth factor in the presence of cycloheximide, was screened to identify RNA transcripts whose abundance is specifically increased as a primary response to growth stimulation. Approximately 40% of the inducible clones detected by this procedure corresponded to either cytoskeletal beta- or gamma-actin genes. One nonactin clone, designated c99, was found to be derived from an 8.5-kilobase RNA whose abundance began to increase as early as 30 min after stimulation. DNA sequencing established the identity of this RNA as fibronectin. Several additional mitogens were then tested and found to efficiently induce fibronectin mRNA. These included fetal calf serum, platelet-derived growth factor, and transforming growth factor type beta. For at least one inducer, fetal calf serum, the increase in mRNA was preceded by an increase in fibronectin gene transcription. This increase was rapid, reaching maximal levels within 10 min, and was accompanied by near-coordinate increases in both c-fos and beta-actin transcription. These results indicate that fibronectin is a member of a class of "early-response" genes, typified by c-fos and including beta-actin, whose rapid expression may be important in mediating cellular responses to peptide growth factors.

MeSH Terms
Actins/biosynthesis Amino Acid Sequence Animals Base Sequence Cell Division/drug effects Cells, Cultured Fibroblasts/drug effects,metabolism Fibronectins/biosynthesis,genetics Gene Expression Regulation/drug effects Growth Substances/pharmacology Mice Mice, Inbred AKR Mitogens/pharmacology Molecular Sequence Data Proto-Oncogene Proteins/biosynthesis Proto-Oncogene Proteins c-fos RNA, Messenger/biosynthesis Stimulation, Chemical Transcription, Genetic
Chemicals
Actins Fibronectins Growth Substances Mitogens Proto-Oncogene Proteins Proto-Oncogene Proteins c-fos RNA, Messenger
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Blatti S P
Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, MN 55905.
Foster D N
Ranganathan G
Moses H L
Getz M J
References (44)
44 references, click to expand
  1. Identification of high-affinity phorbol ester receptor in cytosol of EL4 thymoma cells: requirement for calcium, magnesium, and phospholipids.
    Proc Natl Acad Sci U S A. 1983 May;80(9):2642-6 PMID: 6302698
  2. Xenopus cytoskeletal actin and human c-fos gene promoters share a conserved protein-binding site.
    EMBO J. 1987 Mar;6(3):667-73 PMID: 3582369
  3. Cell surface interactions with extracellular materials.
    Annu Rev Biochem. 1983;52:761-99 PMID: 6351729
  4. Human actin genes are single copy for alpha-skeletal and alpha-cardiac actin but multicopy for beta- and gamma-cytoskeletal genes: 3' untranslated regions are isotype specific but are conserved in evolution.
    Mol Cell Biol. 1983 Oct;3(10):1783-91 PMID: 6646124
  5. Three different fibronectin mRNAs arise by alternative splicing within the coding region.
    Cell. 1983 Dec;35(2 Pt 1):421-31 PMID: 6317187
  6. Cell-specific regulation of the c-myc gene by lymphocyte mitogens and platelet-derived growth factor.
    Cell. 1983 Dec;35(3 Pt 2):603-10 PMID: 6606489
  7. Structure and expression of mouse VL30 genes.
    Mol Cell Biol. 1983 Dec;3(12):2221-31 PMID: 6318090
  8. Differential effects of epidermal growth factor, transforming growth factor, and insulin on DNA and protein synthesis and morphology in serum-free cultures of AKR-2B cells.
    Cancer Res. 1984 Feb;44(2):710-6 PMID: 6362853
  9. Human fibronectin: cell specific alternative mRNA splicing generates polypeptide chains differing in the number of internal repeats.
    Nucleic Acids Res. 1984 Jul 25;12(14):5853-68 PMID: 6462919
  10. Stimulation of 3T3 cells induces transcription of the c-fos proto-oncogene.
    Nature. 1984 Oct 4-10;311(5985):433-8 PMID: 6090941
  11. Changes in levels of actin and tubulin mRNAs upon the lectin activation of lymphocytes.
    Mol Cell Biol. 1984 Sep;4(9):1754-60 PMID: 6548547
  12. Growth inhibitor from BSC-1 cells closely related to platelet type beta transforming growth factor.
    Science. 1984 Nov 9;226(4675):705-7 PMID: 6093254
  13. Specific stimulation of actin gene transcription by epidermal growth factor and cycloheximide.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7476-80 PMID: 6334309
  14. Induction of c-fos gene and protein by growth factors precedes activation of c-myc.
    Nature. 1984 Dec 20-1985 Jan 2;312(5996):716-20 PMID: 6334806
  15. Identification and isolation of a 140 kd cell surface glycoprotein with properties expected of a fibronectin receptor.
    Cell. 1985 Jan;40(1):191-8 PMID: 3155652
  16. Type beta transforming growth factor: a bifunctional regulator of cellular growth.
    Proc Natl Acad Sci U S A. 1985 Jan;82(1):119-23 PMID: 3871521
  17. Type beta transforming growth factor/growth inhibitor stimulates entry of monolayer cultures of AKR-2B cells into S phase after a prolonged prereplicative interval.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):4147-51 PMID: 3858871
  18. Interaction of fibronectin with its receptor on platelets.
    Cell. 1985 Sep;42(2):439-48 PMID: 2411420
  19. Altered beta-actin gene expression in phorbol myristate acetate-treated chondrocytes and fibroblasts.
    Mol Cell Biol. 1985 Jun;5(6):1425-33 PMID: 4033660
  20. Transient accumulation of c-fos RNA following serum stimulation requires a conserved 5' element and c-fos 3' sequences.
    Cell. 1985 Oct;42(3):889-902 PMID: 2414012
  21. The cell substrate attachment (CSAT) antigen has properties of a receptor for laminin and fibronectin.
    J Cell Biol. 1985 Dec;101(6):2134-44 PMID: 2933413
  22. Characterization of a 140-kD avian cell surface antigen as a fibronectin-binding molecule.
    J Cell Biol. 1986 Feb;102(2):442-8 PMID: 2935540
  23. Transforming growth factor-beta stimulates the expression of fibronectin and collagen and their incorporation into the extracellular matrix.
    J Biol Chem. 1986 Mar 25;261(9):4337-45 PMID: 3456347
  24. Fibronectins.
    Sci Am. 1986 Jun;254(6):42-51 PMID: 3010451
  25. Adhesion and cytoskeletal organisation of fibroblasts in response to fibronectin fragments.
    EMBO J. 1986 Apr;5(4):665-70 PMID: 3709521
  26. Two different cis-active elements transfer the transcriptional effects of both EGF and phorbol esters.
    Science. 1986 Dec 19;234(4783):1552-7 PMID: 3491428
  27. Cell surface proteins and malignant transformation.
    Biochim Biophys Acta. 1976 Apr 30;458(1):73-107 PMID: 779852
  28. Effect of cell proliferation on levels and diversity of poly(A)-containing mRNA.
    Cell. 1976 Feb;7(2):255 -65 PMID: 954083
  29. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  30. Control of a cell surface major glycoprotein by epidermal growth factor.
    Science. 1977 Aug 19;197(4305):776-8 PMID: 302030
  31. Gene expression in chemically transformed mouse embryo cells: selective enhancement of the expression of C type RNA tumor virus genes.
    Cell. 1977 Aug;11(4):909-21 PMID: 196768
  32. Induction of DNA synthesis in BALB/c 3T3 cells by serum components: reevaluation of the commitment process.
    Proc Natl Acad Sci U S A. 1977 Oct;74(10):4481-5 PMID: 270695
  33. Dual control of cell growth by somatomedins and platelet-derived growth factor.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1279-83 PMID: 312500
  34. Effects of transformation on fibronectin gene expression using cloned fibronectin cDNA.
    J Biol Chem. 1981 Jan 10;256(1):520-5 PMID: 6256355
  35. Determination of nucleotide sequences in DNA.
    Science. 1981 Dec 11;214(4526):1205-10 PMID: 7302589
  36. Variations in expression of mutant beta actin accompanying incremental increases in human fibroblast tumorigenicity.
    Cell. 1982 Feb;28(2):259-68 PMID: 7199389
  37. Fibronectin has a dual role in locomotion and anchorage of primary chick fibroblasts and can promote entry into the division cycle.
    J Cell Biol. 1982 May;93(2):402-10 PMID: 6178746
  38. Dihydrocytochalasin B disorganizes actin cytoarchitecture and inhibits initiation of DNA synthesis in 3T3 cells.
    Cell. 1982 Aug;30(1):253-62 PMID: 6215122
  39. Polyadenylylated RNA complementary to a mouse retrovirus-like multigene family is rapidly and specifically induced by epidermal growth factor stimulation of quiescent cells.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7317-21 PMID: 6296829
  40. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  41. Expression of a set of growth-related immediate early genes in BALB/c 3T3 cells: coordinate regulation with c-fos or c-myc.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1182-6 PMID: 3469660
  42. Cloning and analysis of the promotor region of the human fibronectin gene.
    Proc Natl Acad Sci U S A. 1987 Apr;84(7):1876-80 PMID: 3031656
  43. Regulation of fibronectin and type I collagen mRNA levels by transforming growth factor-beta.
    J Biol Chem. 1987 May 15;262(14):6443-6 PMID: 3471760
  44. Growth-related changes in specific mRNAs of cultured mouse cells.
    Proc Natl Acad Sci U S A. 1983 Jul;80(14):4271-5 PMID: 6576337
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.4.1119
Published
1988-02-00
Pages
1119-23
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279717
Subset
IM
Grants
NCI NIH HHS · CA16816 · United States
NCI NIH HHS · CA33643 · United States
NIGMS NIH HHS · GM25510 · United States
Databases
GENBANK
J03646, M18194, M18195
External Links
PubMed source DOI: 10.1073/pnas.85.4.1119 PMC Full Text View journal details
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