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PMID: 3125419 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.

Molecular and cellular biology ·Vol. 7 ·No. 12 ·1987-12-00 ·Pages 4194-203

Peterson CL, Calame KL

Abstract

We have begun to purify and characterize several proteins which bind to the mouse immunoglobulin heavy-chain enhancer to understand the molecular interactions important for enhancer activity. Three proteins which bind to different sites on the immunoglobulin heavy-chain enhancer have been chromatographically separated and partially purified. One protein binds a site which has not been reported previously and does not bind to other reported protein-binding sites on the immunoglobulin heavy-chain enhancer. Binding-site boundaries for the three partially purified proteins have been precisely mapped by methylation interference, DNase I footprinting, and orthophenanthroline/copper chemical nuclease footprinting. We have also characterized these three proteins with respect to dissociation rate constants.

MeSH Terms
Animals Base Sequence Binding Sites Cell Nucleus/analysis Chromatography, High Pressure Liquid Copper DNA/metabolism DNA-Binding Proteins/isolation & purification,metabolism Deoxyribonuclease I Electrophoresis, Polyacrylamide Gel Enhancer Elements, Genetic Immunoglobulin Heavy Chains/genetics Indicators and Reagents Methylation Mice Molecular Sequence Data Phenanthrolines Plasmacytoma/analysis Protein Binding
Chemicals
DNA-Binding Proteins Immunoglobulin Heavy Chains Indicators and Reagents Phenanthrolines Copper DNA Deoxyribonuclease I 1,10-phenanthroline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Peterson C L
Molecular Biology Institute, University of California at Los Angeles 90024.
Calame K L
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.12.4194-4203.1987
Published
1987-12-00
Pages
4194-203
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368100
Subset
IM
Grants
NCI NIH HHS · CA38571 · United States
NIGMS NIH HHS · GM29361 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.12.4194-4203.1987 PMC Full Text View journal details
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