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PMID: 3126143 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Iron uptake from lactoferrin and transferrin by Neisseria gonorrhoeae.

Infection and immunity ·Vol. 56 ·No. 4 ·1988-04-00 ·Pages 785-91

McKenna WR, Mickelsen PA, Sparling PF, Dyer DW

Abstract

The major iron (Fe) sources available to Neisseria gonorrhoeae in the human host are probably transferrin (TF) and lactoferrin (LF). Although a number of studies have examined Fe uptake by Neisseria meningitidis, no comparable studies have been done on Fe uptake by the gonococcus from TF and LF. We found that, like meningococci, gonococci removed Fe from TF and LF in an energy-dependent manner; uptake was repressed by Fe and did not proceed by a siderophore-mediated uptake system. Unlike published studies examining meningococcal Fe uptake from TF, our study showed that gonococcal Fe uptake from both TF and LF was highly efficient; uptake saturated at 1 microM protein, and growth with 5% saturated TF and LF occurred at maximal rates when the protein was present in appreciable concentrations. We conclude that the availability of protein-bound Fe probably does not limit growth of N. gonorrhoeae in the human body.

MeSH Terms
Biological Transport Carrier Proteins/metabolism Iron/metabolism Iron-Binding Proteins Lactoferrin/metabolism Lactoglobulins/metabolism Neisseria gonorrhoeae/growth & development,metabolism Transferrin/metabolism Transferrin-Binding Proteins
Chemicals
Carrier Proteins Iron-Binding Proteins Lactoglobulins Transferrin Transferrin-Binding Proteins Iron Lactoferrin
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
McKenna W R
Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill 27599.
Mickelsen P A
Sparling P F
Dyer D W
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.56.4.785-791.1988
Published
1988-04-00
Pages
785-91
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC259371
Subset
IM
Grants
NIAID NIH HHS · AI07001 · United States
NIAID NIH HHS · AI15036 · United States
NIAID NIH HHS · AI23357 · United States
External Links
PubMed source DOI: 10.1128/iai.56.4.785-791.1988 PMC Full Text View journal details
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