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PMID: 3112120 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Aerobactin utilization by Neisseria gonorrhoeae and cloning of a genomic DNA fragment that complements Escherichia coli fhuB mutations.

Journal of bacteriology ·Vol. 169 ·No. 8 ·1987-08-00 ·Pages 3414-21

West SE, Sparling PF

Abstract

Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic DNA with the cloned E. coli aerobactin biosynthesis (iucABCD), aerobactin receptor (iutA), and hydroxamate utilization (fhuCDB) genes. Hybridization was detected with fhuB sequences but not with the other genes under conditions which will detect 70% or greater homology. Similar results were obtained with 21 additional strains of gonococci by colony filter hybridization. A library of DNA from N. gonorrhoeae FA19 was constructed in the phasmid vector lambda SE4, and a clone was isolated that complemented the fhuB mutation in derivatives of E. coli BU736 and BN3307. These results suggest that fhuB is a conserved gene and may play a fundamental role in iron acquisition by N. gonorrhoeae.

MeSH Terms
Bacterial Outer Membrane Proteins Cloacin/pharmacology Cloning, Molecular DNA, Bacterial/genetics Escherichia coli/genetics,metabolism Genes, Bacterial Genetic Complementation Test Hydroxamic Acids/metabolism Iron Chelating Agents/metabolism Mutation Neisseria gonorrhoeae/genetics,metabolism Nucleic Acid Hybridization Receptors, Immunologic/genetics Sequence Homology, Nucleic Acid
Chemicals
Bacterial Outer Membrane Proteins DNA, Bacterial Hydroxamic Acids Iron Chelating Agents Receptors, Immunologic aerobactin receptor aerobactin Cloacin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
West S E
Sparling P F
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.169.8.3414-3421.1987
Published
1987-08-00
Pages
3414-21
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC212411
Subset
IM
Grants
NIAID NIH HHS · AI-07001 · United States
NIAID NIH HHS · AI-15036 · United States
External Links
PubMed source DOI: 10.1128/jb.169.8.3414-3421.1987 PMC Full Text View journal details
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