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PMID: 6317561 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence homology between the immunoglobulin A1 protease genes of Neisseria gonorrhoeae, Neisseria meningitidis, and Haemophilus influenzae.

Infection and immunity ·Vol. 43 ·No. 1 ·1984-01-00 ·Pages 101-7

Koomey JM, Falkow S

Abstract

Isolated DNA fragments encoding the immunoglobulin A1 (IgA1) protease of Neisseria gonorrhoeae were used as hybridization probes to search for homologous sequences in whole cell DNA from Neisseria meningitidis and Haemophilus influenzae. Significant homology was detected. That the detected homology represented IgA1 protease-specific sequences was confirmed by the cloning of these sequences in Escherichia coli HB101 and demonstrating the expression of IgA1 protease by these transformed cells. Molecular probing of commensal Neisseria and Haemophilus species, which do not elaborate IgA1 protease activity, revealed that they were devoid of sequence homology with the cloned IgA1 protease gene DNA.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Bacterial/isolation & purification Genes Genes, Bacterial Haemophilus influenzae/enzymology,genetics Neisseria gonorrhoeae/enzymology,genetics Neisseria meningitidis/enzymology,genetics Nucleic Acid Hybridization Peptide Hydrolases/genetics Plasmids Serine Endopeptidases Species Specificity
Chemicals
DNA, Bacterial DNA Restriction Enzymes Peptide Hydrolases Serine Endopeptidases IgA-specific serine endopeptidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Koomey J M
Falkow S
References (28)
28 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.43.1.101-107.1984
Published
1984-01-00
Pages
101-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC263394
Subset
IM
Grants
NIAID NIH HHS · AI-12192 · United States
External Links
PubMed source DOI: 10.1128/iai.43.1.101-107.1984 PMC Full Text View journal details
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