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PMID: 3127829 Published · ppublish English Journal Article

Active suppression of major histocompatibility complex class II gene expression during differentiation from B cells to plasma cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 7 ·1988-04-00 ·Pages 2229-33

Latron F, Jotterand-Bellomo M, Maffei A, Scarpellino L, Bernard M, Strominger JL, Accolla RS

Abstract

Constitutive expression of major histocompatibility complex class II genes is acquired very early in B-cell ontogeny and is maintained up to the B-cell blast stage. Terminal differentiation in plasma cells is, however, accompanied by a loss of class II gene expression. In B cells this gene system is under the control of several loci encoding transacting factors with activator function, one of which, the aIr-1 gene product, operates across species barriers. In this report human class II gene expression is shown to be extinguished in somatic cell hybrids between the human class II-positive B-cell line Raji and the mouse class II-negative plasmacytoma cell line P3-U1. Since all murine chromosomes are retained in these hybrids and no preferential segregation of a specific human chromosome is observed, the results are compatible with the presence of suppressor factors of mouse origin, operating across species barriers and inhibiting class II gene expression. Suppression seems to act at the level of transcription or accumulation of class II-specific mRNA, since no human, and very few murine, class II transcripts are detectable in the hybrids.

MeSH Terms
Animals B-Lymphocytes/cytology Burkitt Lymphoma/pathology Cell Differentiation Gene Expression Regulation Genes, MHC Class II Histocompatibility Antigens Class II/biosynthesis,genetics Humans Hybrid Cells/metabolism Mice Phenotype Plasma Cells/cytology Plasmacytoma/pathology Transcription Factors/metabolism Tumor Cells, Cultured/pathology
Chemicals
Histocompatibility Antigens Class II Transcription Factors
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Latron F
Ludwig Institute for Cancer Research, Lausanne Branch, Epalinges, Switzerland.
Jotterand-Bellomo M
Maffei A
Scarpellino L
Bernard M
Strominger J L
Accolla R S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.7.2229
Published
1988-04-00
Pages
2229-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC279963
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.7.2229 PMC Full Text View journal details
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