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PMID: 3133442 Published · ppublish English Journal Article

Activation of rat T lymphocytes by anti-CD2 monoclonal antibodies.

The Journal of experimental medicine ·Vol. 167 ·No. 6 ·1988-06-01 ·Pages 1861-72

Clark SJ, Law DA, Paterson DJ, Puklavec M, Williams AF

Abstract

Rat T cells and thymocytes were induced to proliferate by a pair of mAbs, MRC OX-54 and MRC OX-55, directed against rat CD2. Accessory cells were required but their role was not simply for crosslinking of the two mAbs, as neither MRC OX-54 nor MRC OX-55 alone, in the presence of a crosslinking second antibody, caused T cell mitogenesis. Nor could the phorbol ester PMA replace either antibody. The two mAbs recognized distinct epitopes on rat CD2; however, MRC OX-54 could partially block MRC OX-55 binding whereas the reverse situation was not seen. A further CD2 epitope was recognized by two mutually competitive mAbs, MRC OX-34 and MRC OX-53, which were not mitogenic. Neither MRC OX-34 nor MRC OX-53 affected the binding of MRC OX-54 or MRC OX-55, yet they prevented the mitogenic effect induced by these mAbs. The presence of mAbs against CD4 and the IL-2-R also abrogated this mitogenesis, whereas an anti-CD5 mAb augmented the CD2-induced proliferation.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigen-Antibody Reactions Antigen-Presenting Cells/immunology Antigens, Differentiation, T-Lymphocyte/immunology Cells, Cultured Dose-Response Relationship, Immunologic Lymph Nodes/immunology Lymphocyte Activation/drug effects Rats Receptors, Immunologic/immunology Receptors, Interleukin-2 T-Lymphocytes/immunology Tetradecanoylphorbol Acetate/pharmacology
Chemicals
Antibodies, Monoclonal Antigens, Differentiation, T-Lymphocyte Receptors, Immunologic Receptors, Interleukin-2 Tetradecanoylphorbol Acetate
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Clark S J
Medical Research Council Cellular Immunology Research Unit, Sir William Dunn School of Pathology, Oxford, England.
Law D A
Paterson D J
Puklavec M
Williams A F
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.167.6.1861
Published
1988-06-01
Pages
1861-72
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2189691
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.167.6.1861 PMC Full Text View journal details
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