Home LiteratureArticle Details
PMID: 3157987 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and sequencing of cDNA encoding the complete mouse liver alcohol dehydrogenase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 8 ·1985-04-00 ·Pages 2262-6

Edenberg HJ, Zhang K, Fong K, Bosron WF, Li TK

Abstract

The main ethanol-active alcohol dehydrogenase (ADH; alcohol:NAD+ oxidoreductase, EC 1.1.1.1) in mouse liver (ADH-AA) is similar in catalytic and molecular properties to horse liver ADH-EE and to the human class I ADHs. We have isolated cDNA clones encoding the entire mouse liver enzyme plus flanking regions. A mixture of 16 different oligonucleotides, each 14 bases long, was used to screen a liver cDNA library made from a DBA/2J mouse. A strongly hybridizing clone was found and identified as an ADH-encoding cDNA by partial DNA sequencing. This clone was used as a probe to identify others. Two overlapping cDNA clones together contained the entire protein-encoding region plus 100 nucleotides of the 5' noncoding region and 133 nucleotides of the 3' noncoding region culminating in a short poly(dA) tail. The amino acid sequence of the mouse liver enzyme deduced from this cDNA closely resembles that of horse liver ADH-E: 316 of 374 residues are identical, and 29 of the differences are conservative substitutions. The 5' region of this cDNA is interesting: the AUG that initiates the ADH polypeptide is preceded by an AUG that would encode the first amino acid of a tripeptide. Presumably termination of this tripeptide is followed by reinitiation at the AUG immediately preceding the sequence of the mature ADH polypeptide.

MeSH Terms
Alcohol Dehydrogenase Alcohol Oxidoreductases/genetics Amino Acid Sequence Animals Base Sequence Biological Evolution Cloning, Molecular Codon/genetics DNA/genetics Humans Liver/enzymology Mice Species Specificity
Chemicals
Codon DNA Alcohol Oxidoreductases Alcohol Dehydrogenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Edenberg H J
Zhang K
Fong K
Bosron W F
Li T K
References (31)
31 references, click to expand
  1. Some catalytic properties of human liver alcohol dehydrogenase.
    Biochemistry. 1966 Jun;5(6):2026-34 PMID: 5963446
  2. A membrane-filter technique for the detection of complementary DNA.
    Biochem Biophys Res Commun. 1966 Jun 13;23(5):641-6 PMID: 5963888
  3. Horse liver alcohol dehydrogenase. The primary structure of the protein chain of the ethanol-active isoenzyme.
    Eur J Biochem. 1970 Sep;16(1):25-40 PMID: 4917230
  4. Developmental changes and polymorphism in human alcohol dehydrogenase.
    Ann Hum Genet. 1971 Feb;34(3):251-71 PMID: 5548434
  5. Heterogeneity and polymorphism of human-liver alcohol dehydrogenase.
    Eur J Biochem. 1971 Dec;24(2):271-9 PMID: 5157297
  6. Heterogeneity of alcohol dehydrogenase from human liver.
    Arch Biochem Biophys. 1972 Nov;153(1):279-93 PMID: 4405525
  7. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  8. Three-dimensional structure of horse liver alcohol dehydrogenase at 2-4 A resolution.
    J Mol Biol. 1976 Mar 25;102(1):27-59 PMID: 178875
  9. A simple method for DNA restriction site mapping.
    Nucleic Acids Res. 1976 Sep;3(9):2387-98 PMID: 787937
  10. Nucleotide sequences from the rabbit beta globin gene inserted into Escherichia coli plasmids.
    Science. 1977 Jan 28;195(4276):389-91 PMID: 318762
  11. Differences between alcohol dehydrogenases. Structural properties and evolutionary aspects.
    Eur J Biochem. 1977 Feb;72(3):443-52 PMID: 320001
  12. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  13. Sequence of the gene for iso-1-cytochrome c in Saccharomyces cerevisiae.
    Cell. 1979 Apr;16(4):753-61 PMID: 222467
  14. A rapid alkaline extraction procedure for screening recombinant plasmid DNA.
    Nucleic Acids Res. 1979 Nov 24;7(6):1513-23 PMID: 388356
  15. Filter replicas and permanent collections of recombinant DNA plasmids.
    Nucleic Acids Res. 1979 Dec 20;7(8):2115-36 PMID: 118435
  16. Cloning of hormone genes from a mixture of cDNA molecules.
    Methods Enzymol. 1979;68:75-90 PMID: 232226
  17. The use of synthetic oligonucleotides as hybridization probes. II. Hybridization of oligonucleotides of mixed sequence to rabbit beta-globin DNA.
    Nucleic Acids Res. 1981 Feb 25;9(4):879-94 PMID: 7232206
  18. In vivo chemical modification of proteins (post-translational modification).
    Annu Rev Biochem. 1981;50:783-814 PMID: 6791580
  19. A comprehensive sequence analysis program for the IBM personal computer.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):581-99 PMID: 6546431
  20. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  21. Purification and characterization of mouse alcohol dehydrogenase from two inbred strains that differ in total liver enzyme activity.
    Biochem Genet. 1984 Feb;22(1-2):115-24 PMID: 6370228
  22. Initiation of translation at internal AUG codons in mammalian cells.
    Nature. 1984 May 3-9;309(5963):82-5 PMID: 6717585
  23. Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
    Nature. 1984 May 10-16;309(5964):179-82 PMID: 6325940
  24. Human liver alcohol dehydrogenase: amino acid substitution in the beta 2 beta 2 Oriental isozyme explains functional properties, establishes an active site structure, and parallels mutational exchanges in the yeast enzyme.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3024-8 PMID: 6374651
  25. Molecular cloning and characterization of a cDNA for the beta subunit of human alcohol dehydrogenase.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4055-9 PMID: 6330735
  26. Apple II software for M13 shotgun DNA sequencing.
    Nucleic Acids Res. 1982 Jan 11;10(1):39-49 PMID: 6278410
  27. Cloning and sequence analysis of cDNA for porcine beta-neo-endorphin/dynorphin precursor.
    Nature. 1982 Jul 15;298(5871):245-9 PMID: 6123953
  28. Kinetic and electrophoretic properties of native and recombined isoenzymes of human liver alcohol dehydrogenase.
    Biochemistry. 1983 Apr 12;22(8):1852-7 PMID: 6342668
  29. New M13 vectors for cloning.
    Methods Enzymol. 1983;101:20-78 PMID: 6310323
  30. Relationship between kinetics of liver alcohol dehydrogenase and alcohol metabolism.
    Pharmacol Biochem Behav. 1983;18 Suppl 1:223-7 PMID: 6356161
  31. Purification and molecular properties of mouse alcohol dehydrogenase isozymes.
    Eur J Biochem. 1983 Dec 1;137(1-2):139-47 PMID: 6360682
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.8.2262
Published
1985-04-00
Pages
2262-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397537
Subset
IM
Grants
NIAAA NIH HHS · AA02342 · United States
NIAAA NIH HHS · AA04307 · United States
NIAAA NIH HHS · AA06460 · United States
Databases
GENBANK
M11307
External Links
PubMed source DOI: 10.1073/pnas.82.8.2262 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2