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PMID: 3162159 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

A convenient technique to compare the efficiency of promoters in Escherichia coli.

Nucleic acids research ·Vol. 13 ·No. 16 ·1985-08-26 ·Pages 5919-26

Vidal-Ingigliardi D, Raibaud O

Abstract

We describe a technique which allows one to insert any promoter in front of the chromosomal malPQ operon. This can be done easily by using only one plasmid, one strain, and two simple selections. Properties of the final chromosomal fusion are such that the level of amylomaltase, the product of the malQ gene, measures quantitatively the efficiency of the inserted promoter. This method was utilized to compare the efficiency of four well-known promoters: lacZp, trp, tac, lambdaPR and three malT activated promoters: malPp, malkP and malEp.

MeSH Terms

Chromosomes, Bacterial Escherichia coli/genetics Genotype Glucosyltransferases/genetics Glycogen Debranching Enzyme System Operon Plasmids Species Specificity Transcription, Genetic

Chemicals

Glycogen Debranching Enzyme System Glucosyltransferases 4 alpha-glucanotransferase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Vidal-Ingigliardi D

Raibaud O

References (26)

26 references, click to expand

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Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/13.16.5919

Published

1985-08-26

Pages

5919-26

Language

English

Region

England

NLM ID

0411011

PMCID

PMC321922

Subset

External Links

PubMed source DOI: 10.1093/nar/13.16.5919 PMC Full Text View journal details

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A convenient technique to compare the efficiency of promoters in Escherichia coli.

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