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PMID: 316538 Published · ppublish English Journal Article

Human lymphocyte antigens: production of a monoclonal antibody that defines functional thymus-derived lymphocyte subsets.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 11 ·1979-11-00 ·Pages 5829-33

Haynes BF, Eisenbarth GS, Fauci AS

Abstract

A monoclonal mouse antibody (3A1) that specifically bound to 65% of human peripheral blood (PB) thymus-derived (T) cells but did not bind to complement receptor-positive PB bone marrow-derived (B) cells, polymorphonuclear leukocytes, or human erythrocytes has been produced. The 3AI antibody was synthesized by a stable cloned lymphocyte hybrid cell line. This lymphocyte hybrid line (3AI) was derived from fusion of P3 X 63/Ag8 myeloma cells and spleen cells from BALB/c mice immunized with HSB-2 cells, a human T cell line. The 3A1 lymphocyte hybrid line produced mouse ascites fluid containing 3A1 antibody in saturating titers of up to 1:25,600. Purified PB T cells that carried the 3A1 antigen incorporated tritiated thymidine maximally in response to phytohemagglutinin and concanavalin A stimulation, whereas purified PB T cells that lacked the 3A1 antigen responded suboptimally to phytohemagglutinin and minimally to concanavalin A. Thus, the 3A1 antibody can be easily used to study the role of 3A1-positive and negative T cell subsets in the regulation of normal and abnormal human immune responses.

MeSH Terms
Animals Antibody Specificity Antigen-Antibody Reactions Antigens, Surface/analysis Clone Cells/immunology Female Humans Isoantibodies/isolation & purification Isoantigens/analysis Lymphocyte Activation Mice Rosette Formation T-Lymphocytes/classification,immunology
Chemicals
Antigens, Surface Isoantibodies Isoantigens
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Haynes B F
Eisenbarth G S
Fauci A S
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.11.5829
Published
1979-11-00
Pages
5829-33
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC411745
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.11.5829 PMC Full Text View journal details
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