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PMID: 318655 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Fractionation of Saccharomyces cerevisiae cell populations by centrifugal elutriation.

Journal of bacteriology ·Vol. 129 ·No. 1 ·1977-01-00 ·Pages 97-100

Gordon CN, Elliott SC

Abstract

An exponential population of Saccharomyces cerevisiae cells was fractionated by centrifugal elutriation, using water as the elutriating liquid. Evidence that the population had been fractionated according to age in the cell cycle was obtained by examining the fractions for their size distribution, their microscopic appearance after Giemsa staining, and their ability to initiate synchronous growth.

MeSH Terms
Cell Division Cell Separation/methods Centrifugation Saccharomyces cerevisiae/cytology,growth & development
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gordon C N
Elliott S C
References (6)
6 references, click to expand
  1. Cell cycle analysis by culture fractionation.
    Exp Cell Res. 1972 Dec;75(2):465-74 PMID: 4566304
  2. Saccharomyces cerevisiae cell cycle.
    Bacteriol Rev. 1974 Jun;38(2):164-98 PMID: 4599449
  3. Periodic density fluctuation during the yeast cell cycle and the selection of synchronous cultures.
    J Bacteriol. 1970 Dec;104(3):1280-5 PMID: 16559104
  4. Macromolecule synthesis in temperature-sensitive mutants of yeast.
    J Bacteriol. 1967 May;93(5):1662-70 PMID: 5337848
  5. Separation of biological particles by centrifugal elutriation.
    Anal Biochem. 1968 Jun;23(3):369-77 PMID: 5658098
  6. Use of yeast populations fractionated by zonal centrifugation to study the cell cycle.
    J Bacteriol. 1971 Dec;108(3):1045-50 PMID: 4945182
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.129.1.97-100.1977
Published
1977-01-00
Pages
97-100
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC234900
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.129.1.97-100.1977 PMC Full Text View journal details
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