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PMID: 3208739 Published · ppublish English Journal Article

Nuclear scaffold attachment sites in the human globin gene complexes.

The EMBO journal ·Vol. 7 ·No. 11 ·1988-11-00 ·Pages 3337-44

Jarman AP, Higgs DR

Abstract

In an analysis of a 90-kb region around the human beta-globin gene complex we have identified at least eight sites of attachment to the nuclear scaffold (SARs). While these have many potential functions, there appears to be a particular association with sequences important in the regulation of the complex. Two SARs are close to the known enhancer-like elements of the beta-globin gene. SARs flanking the complex co-habit with the boundaries of the putative beta-like globin gene regulatory domain. In contrast, we have detected no SARs within a 140-kb region of the human alpha-globin gene complex. If SARs play a role in the regulation of gene expression then this structural difference would imply a difference in the regulation of the two complexes.

MeSH Terms
Binding Sites Blotting, Southern Cell Nucleus/metabolism DNA/analysis,genetics Gene Expression Regulation Globins/genetics Humans Multigene Family Nucleic Acid Hybridization Restriction Mapping
Chemicals
Globins DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jarman A P
Nuffield Department of Clinical Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
Higgs D R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-11-00
Pages
3337-44
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454829
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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