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PMID: 3208740 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

RNA binding specificity of hnRNP proteins: a subset bind to the 3' end of introns.

The EMBO journal ·Vol. 7 ·No. 11 ·1988-11-00 ·Pages 3519-29

Swanson MS, Dreyfuss G

Abstract

The binding of hnRNP proteins to pre-mRNAs in nuclear extracts, and as isolated proteins, was studied by using monoclonal antibody immunopurification of hnRNP proteins bound to RNase T1-generated fragments. Several major hnRNP proteins, A1, C and D, bind specifically to the 3' end of introns within a region containing the conserved polypyrimidine stretch between the branch site and the 3' splice site. Mutations which alter the conserved 3' splice site dinucleotide AG strongly impair or abolish the binding of the A1 protein as well as of an anti-Sm reactive component(s) to this region. The A1, C and D proteins do not bind efficiently to fragments of either bacterial RNA or the intronless spliced product (mRNA). The binding of these proteins at the 3' end of the intron does not require addition to the extract of exogenous ATP, but remains after ATP addition. These findings demonstrate that several hnRNP proteins have RNA binding specificities on pre-mRNA, and suggest a model for hnRNP particle structure and assembly.

MeSH Terms
Binding Sites Electrophoresis, Polyacrylamide Gel HeLa Cells Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins Humans Introns Mutation Precipitin Tests RNA Precursors/metabolism RNA Splicing RNA, Messenger/metabolism Ribonucleoproteins/metabolism
Chemicals
Heterogeneous Nuclear Ribonucleoprotein A1 Heterogeneous-Nuclear Ribonucleoprotein Group A-B Heterogeneous-Nuclear Ribonucleoproteins RNA Precursors RNA, Messenger Ribonucleoproteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Swanson M S
Department of Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston, IL 60208.
Dreyfuss G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1988-11-00
Pages
3519-29
Language
English
Region
England
NLM ID
8208664
PMCID
PMC454853
Subset
IM
Grants
NIGMS NIH HHS · GM-31888 · United States
NIGMS NIH HHS · GM-37125 · United States
External Links
PubMed source PMC Full Text View journal details
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