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PMID: 3215997 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Length and myofilament spacing-dependent changes in calcium sensitivity of skeletal fibres: effects of pH and ionic strength.

Journal of muscle research and cell motility ·Vol. 9 ·No. 5 ·1988-10-00 ·Pages 428-45

Martyn DA, Gordon AM

Abstract

The calcium sensitivity of force was measured in glycerinated rabbit psoas fibres at sarcomere lengths (SL) from 2.3 to 3.4 micron. Increased SL caused calcium sensitivity to increase and the slope of force-calcium relations to decrease. We have hypothesized that length-dependent changes in myofilament lattice spacing and the presence of fixed charge on the myofilaments are important in determining calcium sensitivity. Lattice spacing changes were monitored by measuring fibre diameter (D). D was decreased by increasing SL, decreasing bathing solution pH and by osmotic compression with 3% PVP. 3% PVP caused D to decrease by about 15% at all SLs and pH values tested. Force-calcium relations were measured at different SLs and pH values, with and without 3% PVP in the bathing solutions. At all pH values D at SL 2.3 micron with 3% PVP was comparable to the value at 3.4 micron, without PVP. At pH 7.5 and 7.0 calcium sensitivity was about the same at both SL, although the slope of the force-calcium relation was less at longer SL. The similarity of the calcium sensitivity at the same D, but much different SL, indicates that lattice spacing is important in determining calcium sensitivity, while SL and the degree of myofilament overlap are important in determining the slope of force-calcium relations. In order to test for the role of myofilament charge in determining calcium sensitivity, pH and ionic strength were varied. Decreasing pH caused decreased maximum force and calcium sensitivity. In addition, the influence of SL on calcium sensitivity decreased as pH was lowered, with minimal SL dependence at pH 5.5; even though lattice spacing still decreased with increasing SL. When D was decreased with PVP, calcium sensitivity increased at all SLs in pH 7.5 and 7.0 while the same lattice spacing changes at pH 6.0 and 5.5 resulted in greatly reduced shifts in calcium sensitivity. These results indicate that the effect of lattice spacing on calcium sensitivity depends on myofilament charge. At pH 6.0, even though osmotic compression of the lattice has no effect, increasing SL causes about half the shift in calcium sensitivity seen at pH 7.0. Lowering ionic strength from 200 to 110 mM caused an increase in both the magnitude and length dependence of calcium sensitivity at pH 7.0, while at pH 5.5 both decreased.(ABSTRACT TRUNCATED AT 400 WORDS)

MeSH Terms
Actin Cytoskeleton/drug effects,ultrastructure Animals Calcium/pharmacology Cytoskeleton/ultrastructure Hydrogen-Ion Concentration Muscles/drug effects,ultrastructure Osmolar Concentration Rabbits Sarcomeres/drug effects,ultrastructure
Chemicals
Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Martyn D A
Center for Bioengineering, University of Washington, Seattle 98195.
Gordon A M
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Article Info
Journal
Journal of muscle research and cell motility
Abbr.
J Muscle Res Cell Motil
ISSN
0142-4319
DOI
10.1007/BF01774069
Published
1988-10-00
Pages
428-45
Language
English
Region
Netherlands
NLM ID
8006298
Subset
IM
Grants
NHLBI NIH HHS · HL 31962 · United States
NINDS NIH HHS · NS 08384 · United States
External Links
PubMed source DOI: 10.1007/BF01774069 View journal details
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