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PMID: 322146 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction between mutations of ribosomes and RNA polymerase: a pair of strA and rif mutants individually temperature-insensitive but temperature-sensitive in combination.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 3 ·1977-03-00 ·Pages 1157-61

Chakrabarti SL, Gorini L

Abstract

A temperature-sensitive lethal mutant of Escherichia coli has been constructed by combining two temperature-insensitive mutations: a rif180 mutation that modifies RNA polymerase (RNA nucleotidyltransferase; nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2.7.7.6) and a strA24 mutation that modifies the ribosomal protein S12. The temperature sensitivity is a property of the combination of these two particular alleles; replacement of either of the alleles relieves the temperature sensitivity. An isogenic strain containing a different strA mutation (i.e., rif180 strA11) is not temperature sensitive. Evidently ribosomes modified by the particular strA24 polymerase altered by the rif180 mutation, which suggests that in vivo there may exist some interaction between structures of ribosomes and the RNA polymerase.

MeSH Terms
Bacterial Proteins/biosynthesis DNA-Directed RNA Polymerases/metabolism Escherichia coli Genotype Mutation Phenotype Ribosomal Proteins/physiology Ribosomes/physiology Rifampin/pharmacology Streptomycin/pharmacology Temperature Transduction, Genetic
Chemicals
Bacterial Proteins Ribosomal Proteins DNA-Directed RNA Polymerases Rifampin Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chakrabarti S L
Gorini L
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.3.1157
Published
1977-03-00
Pages
1157-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC430631
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.3.1157 PMC Full Text View journal details
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