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PMID: 4365378 Published · ppublish English Journal Article

The synthesis in vitro of RNA polymerase subunits of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 8 ·1973-08-00 ·Pages 2420-3

Austin S, McGeoch D

Abstract

DNA from transducing bacteriophage that carry the structural gene for the beta subunit of RNA polymerase has been used to direct protein synthesis in a cell-free transcription-translation system. A polypeptide species has been detected which appears to be beta-subunit material made in vitro.

MeSH Terms
Cell-Free System Coliphages/analysis Cyclic AMP/pharmacology DNA, Viral/isolation & purification,metabolism DNA-Directed RNA Polymerases/analysis,biosynthesis Electrophoresis, Polyacrylamide Gel Escherichia coli/enzymology Molecular Weight Protein Biosynthesis Templates, Genetic Transcription, Genetic Tritium
Chemicals
DNA, Viral Tritium Cyclic AMP DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Austin S
McGeoch D
References (19)
19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.8.2420
Published
1973-08-00
Pages
2420-3
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433748
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.8.2420 PMC Full Text View journal details
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