In vitro transcription of three different ribosomal RNA cistrons of E. coli; heterogeneity of control regions.

Molecular & general genetics : MGG ·Vol. 152 ·No. 1 ·1977-03-28 ·Pages 1-6

Oostra BA, van Ooyen AJ, Gruber M

Abstract

Ribosomal RNA synthesis from three different rRNA cistrons of E. coli, located on different phage DNAs was compared and found to have the same characteristics as regards chain length, salt and temperature dependence and the effect of ppGpp. However, some clear and reproducible quantitative differences between rRNA synthesis from the different templates both in presence and absence of ppGGpp were found. Rifampicin and heparin experiments showed that these differences were located at the initiation sites. We propose that heterogeneity exists in the RNA polymerase binding regions of the rRNA prmoters in E. coli.

MeSH Terms

Coliphages/metabolism DNA, Bacterial/metabolism Escherichia coli/metabolism Peptide Chain Initiation, Translational Potassium Chloride/pharmacology RNA, Ribosomal/biosynthesis Rifampin/pharmacology Temperature Templates, Genetic Transcription, Genetic

Chemicals

DNA, Bacterial RNA, Ribosomal Potassium Chloride Rifampin

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Oostra B A

van Ooyen A J

Gruber M

References (26)

26 references, click to expand

Preparation of guanosine tetraphosphate (ppGpp) and guanosine pentaphosphate (pppGpp) from Escherichia coli ribosomes.
Anal Biochem. 1974 Jan;57(1):100-7 PMID: 4593930
Influence of salts on RNA synthesis by DNA-dependent RNA-polymerase from Escherichia coli.
Eur J Biochem. 1967 Dec;3(2):183-93 PMID: 4865567
Two compounds implicated in the function of the RC gene of Escherichia coli.
Nature. 1969 Mar 1;221(5183):838-41 PMID: 4885263
Interaction of rifamycin with bacterial RNA polymerase.
Proc Natl Acad Sci U S A. 1968 Oct;61(2):667-73 PMID: 4879400
A ribosomal RNA gene of Escherichia coli (rrnD) on lamnda daro E specialized transducing phages.
Mol Gen Genet. 1976 Aug 2;146(3):303-7 PMID: 794695
Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. 3. The effect of temperature on ribonucleic acid chain initiation and on the conformation of binary complexes.
J Biol Chem. 1974 May 25;249(10):3007-13 PMID: 4598118
Termination factor for RNA synthesis.
Nature. 1969 Dec 20;224(5225):1168-74 PMID: 4902144
The mechanism of action of ppGpp on rRNA synthesis in vitro.
Cell. 1976 May;8(1):123-8 PMID: 8211
Transcription in vitro of DNA in isolated bacterial nucleoids.
J Mol Biol. 1975 Aug 5;96(2):217-37 PMID: 1100843
Heterogeneous initiation and termination of enzymically synthesized ribonucleic acid.
J Mol Biol. 1969 Jul 14;43(1):89-107 PMID: 4897796
Further mapping of 5S RNA cistrons in Escherichia coli.
Mol Gen Genet. 1973 Oct 16;126(1):29-35 PMID: 4591367
Synthesis of ribosomal RNA on a protein-DNA complex isolated from bacteria: a comparison of ribosomal RNA synthesis in vitro and in vivo.
J Mol Biol. 1970 Sep 14;52(2):281-300 PMID: 4991980
A rapid and sensitive determination of bacterial rRNA by means of hybridization-competition.
Anal Biochem. 1976 Aug;74(2):496-502 PMID: 786069
Transcription of Azotobacter phage deoxyribonucleic acid. Salt-dependent equilibrium between steps in initiation.
J Biol Chem. 1975 Apr 25;250(8):2872-7 PMID: 1091643
Modulation of RNA polymerase specificity by ppGpp.
Mol Gen Genet. 1976 Aug 19;147(2):225-32 PMID: 787760
Structure and synthesis of the ribosomal ribonucleic acid of prokaryotes.
Bacteriol Rev. 1973 Dec;37(4):562-603 PMID: 4203396
Localization of some 5s RNA cistrons on Escherichia coli chromosome.
Mol Gen Genet. 1973 Mar 1;121(2):151-62 PMID: 4571522
Specific inhibition of ribosomal RNA synthesis in vitro by guanosine 3' diphosphate, 5' diphosphate.
Nature. 1975 Apr 10;254(5500):530-1 PMID: 1091863
A COMPLEX OF ENZYMATICALLY SYNTHESIZED RNA AND TEMPLATE DNA.
Proc Natl Acad Sci U S A. 1964 May;51:801-8 PMID: 14172994
Ribosomal and non-ribosomal RNA synthesis in vitro.
Biochim Biophys Acta. 1975 Jun 16;395(2):128-35 PMID: 1095071
The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. III. Further studies.
Biochimie. 1975;57(6-7):711-48 PMID: 1106773
The 30 S ribosomal precursor RNA from Escherichia coli. A primary transcript containing 23 S, 16 S, and 5 S sequences.
J Biol Chem. 1975 Jul 25;250(14):5647-54 PMID: 1095585
Mode of action of rafamycin on the RNA polymerase reaction.
Biochim Biophys Acta. 1968 Mar 18;157(1):218-9 PMID: 4868249
Studies of ribonucleic acid chain initiation by Escherichia coli ribonucleic acid polymerase bound to T7 deoxyribonucleic acid. II. The effect of alterations in ionic strength of chain initiation and on the conformation of binary complexes.
J Biol Chem. 1974 May 25;249(10):3002-6 PMID: 4598117
Isolation of a specialized lambda transducing bacteriophage carrying the beta subunit gene for Escherichia coli ribonucleic acid polymerase.
J Bacteriol. 1973 Nov;116(2):517-26 PMID: 4583237
Transfer RNA genes between 16S and 23S rRNA genes in rRNA transcription units of E. coli.
Cell. 1976 Feb;7(2):165-77 PMID: 782716

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00264932

Published

1977-03-28

Pages

1-6

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00264932

Analysis Services

In vitro transcription of three different ribosomal RNA cistrons of E. coli; heterogeneity of control regions.

Contact

WeChat Official Account