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PMID: 325555 Published · ppublish English Journal Article

Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 74 ·No. 5 ·1977-05-00 ·Pages 1831-5

Nakamura Y, Osawa T, Yura T

Abstract

A set of F' strains of Escherichia coli K-12 partially diploid for various chromosomal segments has been examined for possible gene dosage effects in the synthesis of sigma factor of the DNA-dependent RNA polymerase (RNA nucleotidyltransferase; nucleoside-triphosphate:RNA nucleoti-dyltransferase, EC 2.7.7.6). It was found that all F' strains diploid for the dnaG region synthesize sigma at rates two to three times higher than other F' or F- strains. Moreover, strains of Salmonella typhimurium harboring these F' plasmids produce E. coli sigma in addition to Salmonella sigma. This has been shown on the basis of the finding that Salmonella sigma can be precipitated with antiserum against E. coli RNA polymerase but is distinguishable from E. coli sigma in its mobility in sodium dodecyl sulfate/polyacrylamide gel electrophoresis. E. coli sigma polypeptides thus produced seem to be stable in cells of S. typhimurium. These results indicate that a structural gene for sigma (rpoD) is located at the metC-argG region, probably near the dnaG locus (66 min on the current genetic map of E. coli).

MeSH Terms
Alleles Chromosome Mapping DNA, Bacterial/metabolism DNA, Recombinant/metabolism DNA-Directed RNA Polymerases/biosynthesis Escherichia coli/metabolism F Factor Genes Genetic Linkage Plasmids Salmonella typhimurium/metabolism Sigma Factor/biosynthesis Transcription Factors/biosynthesis
Chemicals
DNA, Bacterial DNA, Recombinant Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nakamura Y
Osawa T
Yura T
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29 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.74.5.1831
Published
1977-05-00
Pages
1831-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431019
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.74.5.1831 PMC Full Text View journal details
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