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PMID: 794877 Published · ppublish English Journal Article

Induction of sigma factor synthesis in Escherichia coli by the N gene product of bacteriophage lambda.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 12 ·1976-12-00 ·Pages 4405-9

Nakamura Y, Yura T

Abstract

Thermoinduction of cells of E. coli carrying prophage lambdacI857 within the bfe gene brings about not only "escape synthesis" of core subunits of the DNA-dependent RNA polymerase (RNA nucleotidyltransferase, nucleosidetriphosphate:RNA nucleotidyltransferase, EC 2-7-7-6), but also a striking stimulation of sigma factor synthesis. The latter phenomenon, termed sigma induction, is generally observed after lambda phage infection or prophage induction. A series of experiments with various bacterial and phage strains led us to conclude that the N gene product of lambda is directly involved to the sigma induction. These and other results obtained with mutants defective in transcription termination factor rho suggest the involvement of a rho-sensitive site in the control of sigma gene expression in E. coli.

MeSH Terms
Chromosome Mapping Coliphages/growth & development,metabolism DNA-Directed RNA Polymerases/biosynthesis Escherichia coli/enzymology Genes, Regulator Lysogeny Mutation Rho Factor/biosynthesis Sigma Factor/biosynthesis Transcription Factors/biosynthesis Virus Replication
Chemicals
Rho Factor Sigma Factor Transcription Factors DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nakamura Y
Yura T
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31 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.12.4405
Published
1976-12-00
Pages
4405-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431475
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.12.4405 PMC Full Text View journal details
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