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PMID: 3258006 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The essential role of B cell stimulatory factor 2 (BSF-2/IL-6) for the terminal differentiation of B cells.

The Journal of experimental medicine ·Vol. 167 ·No. 2 ·1988-02-01 ·Pages 332-44

Muraguchi A, Hirano T, Tang B, Matsuda T, Horii Y, Nakajima K, Kishimoto T

Abstract

The role of recombinant B cell stimulatory factor 2 (BSF-2/IL-6) in the regulation of growth and differentiation of B cells was investigated. rBSF-2 at 200 pg/ml could induce 50% of the maximum Ig production in B lymphoblastoid cell lines, the specific activity being estimated as 5 X 10(6) U/mg. rBSF-2 augmented PWM-induced IgM, IgG, and IgA production in mononuclear cells (MNC); the effect was exerted by directly acting on PWM-induced B blast cells to induce Ig production. However, rBSF-2 did not induce any growth of activated B cells. In contrast, rBSF-2 showed a potent growth activity on a murine hybridoma clone, MH60.BSF2. The concentration required for half-maximal [3H]TdR uptake was approximately 5 pg/ml, which was 40 times less than that required for Ig induction in a B cell line. Anti-BSF-2 antibody inhibited PWM-induced Ig production in MNC, but not PWM-induced proliferation. The antibody was effective even when added on day 4 of an 8-d culture, indicating that BSF-2 is one of the essential late-acting factors in PWM-induced Ig production.

MeSH Terms
Antibodies/physiology B-Lymphocytes/cytology,metabolism Cell Differentiation/drug effects Cell Line, Transformed Humans Hybridomas/cytology Immunoglobulins/biosynthesis Interleukin-4 Interleukins/immunology,physiology Lymphocyte Activation/drug effects Pokeweed Mitogens/pharmacology Recombinant Proteins/pharmacology
Chemicals
Antibodies Immunoglobulins Interleukins Pokeweed Mitogens Recombinant Proteins Interleukin-4
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Muraguchi A
Division of Immunology, Osaka University, Japan.
Hirano T
Tang B
Matsuda T
Horii Y
Nakajima K
Kishimoto T
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.167.2.332
Published
1988-02-01
Pages
332-44
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2188837
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.167.2.332 PMC Full Text View journal details
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