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PMID: 3260487 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence and comparative analysis of three Enterobacter cloacae ampC beta-lactamase genes and their products.

The Biochemical journal ·Vol. 250 ·No. 3 ·1988-03-15 ·Pages 753-60

Galleni M, Lindberg F, Normark S, Cole S, Honore N, Joris B, Frere JM

Abstract

The sequences of three Enterobacter cloacae ampC beta-lactamase genes have been determined. The deduced amino acid sequences are very similar: out of a total of 361 residues, only eight positions were found to be variable, and several mutations yielded residues with very similar properties. The kinetic properties of two of the enzymes were not significantly different. The three enzymes also exhibited a high degree of homology (greater than 70%) with the ampC beta-lactamases of Escherichia coli K12 and Citrobacter freundii, confirming the homogeneity of class-C beta-lactamases.

MeSH Terms
Amino Acid Sequence Bacterial Proteins Base Sequence Cloning, Molecular Enterobacter/enzymology,genetics Enterobacteriaceae/genetics Genes, Bacterial Kinetics Molecular Sequence Data Peptide Mapping Protein Sorting Signals/analysis beta-Lactamases/genetics
Chemicals
Bacterial Proteins Protein Sorting Signals AmpC beta-lactamases beta-Lactamases
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Galleni M
Université de Liège, Institut de Chimie, Belgium.
Lindberg F
Normark S
Cole S
Honore N
Joris B
Frere J M
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2500753
Published
1988-03-15
Pages
753-60
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1148921
Subset
IM
Databases
GENBANK
X07274, X08081, X08082
External Links
PubMed source DOI: 10.1042/bj2500753 PMC Full Text View journal details
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