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PMID: 3260607 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular characterization of human Ro/SS-A antigen. Amino terminal sequence of the protein moiety of human Ro/SS-A antigen and immunological activity of a corresponding synthetic peptide.

The Journal of clinical investigation ·Vol. 82 ·No. 1 ·1988-07-00 ·Pages 96-101

Lieu TS, Newkirk MM, Capra JD, Sontheimer RD

Abstract

The Ro/SS-A antigen was purified from an Epstein-Barr virus-transformed human B lymphoblastoid cell line. The amino terminal amino acid sequence of the 60-kD polypeptide bearing this antigenic epitope was determined to be: (formula; see text) A peptide composed of residue 6-19 was synthesized by the solid-phase method. Immunodiffusion-defined monospecific autoimmune sera to Ro/SS-A reacted with this synthetic peptide in ELISA, whereas autoantibodies with other specificities such as anti-La/SS-B and anti-Sm, as well as normal human sera, were not reactive. In addition, rabbit anti-peptide 6-19 antisera reacted specifically with native human Ro/SS-A antigen in ELISA. Furthermore, this synthetic peptide inhibited the binding of rabbit anti-peptide antiserum to native human Ro/SS-A. An additional synthetic peptide corresponding to residues 7-24 partially inhibited the binding of a patient anti-Ro/SS-A serum to native Ro/SS-A. These results suggest that the amino terminal portion of the molecule represents a major epitope of Ro/SS-A. The determination of the amino acid sequence of Ro/SS-A and the availability of synthetic peptide(s) bearing this antigen should provide additional approaches to further characterize the autoimmune response to this antigen.

MeSH Terms
Amino Acid Sequence Animals Antigen-Antibody Reactions Autoantigens/immunology,isolation & purification B-Lymphocytes/immunology Cell Line Enzyme-Linked Immunosorbent Assay Humans Immune Sera/immunology Molecular Sequence Data Peptides/chemical synthesis,immunology RNA, Small Cytoplasmic Rabbits Ribonucleoproteins Spectrophotometry, Ultraviolet
Chemicals
Autoantigens Immune Sera Peptides RNA, Small Cytoplasmic RO60 protein, human Ribonucleoproteins SS-A antigen
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lieu T S
Department of Dermatology, University of Texas Health Science Center, Dallas 75235.
Newkirk M M
Capra J D
Sontheimer R D
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113607
Published
1988-07-00
Pages
96-101
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303481
Subset
IM
Grants
NIAID NIH HHS · AI-12127 · United States
NIADDK NIH HHS · AM-19101 · United States
NIGMS NIH HHS · GM-31689 · United States
External Links
PubMed source DOI: 10.1172/JCI113607 PMC Full Text View journal details
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