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PMID: 3263647 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Stepwise intraclonal maturation of antibody affinity through somatic hypermutation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 8206-10

Kocks C, Rajewsky K

Abstract

Using recombinant DNA techniques, we reconstructed a genealogical tree (Sablitzky, F., Wildner, G. & Rajewsky, K. (1985) EMBO J. 4, 345-350) that connects three clonally related B cells producing somatically mutated antibodies to a progenitor cell expressing a germ line-encoded antibody. The somatic mutants had been isolated from an in vivo immune response. The germ line-encoded progenitor antibody bound the antigen with high affinity. Intraclonal affinity maturation occurred stepwise over a 15-fold range.

MeSH Terms
Animals Antibody Specificity B-Lymphocytes/immunology Base Sequence Cell Differentiation Cell Line Clone Cells/immunology DNA, Recombinant/analysis Immunoglobulin Variable Region/immunology Stem Cells/immunology Transfection
Chemicals
DNA, Recombinant Immunoglobulin Variable Region
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kocks C
Institute for Genetics, University of Cologne, Federal Republic of Germany.
Rajewsky K
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40 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.8206
Published
1988-11-00
Pages
8206-10
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282396
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.85.21.8206 PMC Full Text View journal details
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