Home LiteratureArticle Details
PMID: 6312453 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Functional immunoglobulin M production after transfection of cloned immunoglobulin heavy and light chain genes into lymphoid cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 20 ·1983-10-00 ·Pages 6351-5

Ochi A, Hawley RG, Hawley T, Shulman MJ, Traunecker A, Köhler G, Hozumi N

Abstract

The rearranged immunoglobulin heavy (mu) and light (kappa) chain genes cloned from the Sp6 hybridoma cell line producing immunoglobulin M specific for the hapten 2,4, 6-trinitrophenyl were inserted into the transfer vector pSV2-neo and introduced into various plasmacytoma and hybridoma cell lines. The transfer of the mu and kappa genes resulted in the production of pentameric, hapten-specific, functional IgM.

MeSH Terms
Animals Gene Expression Regulation Genetic Vectors Hybridomas/physiology Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Immunoglobulin M/biosynthesis,genetics Immunoglobulin kappa-Chains/genetics Immunoglobulin mu-Chains/genetics Mice Plasmacytoma/genetics Transfection
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Light Chains Immunoglobulin M Immunoglobulin kappa-Chains Immunoglobulin mu-Chains
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Ochi A
Hawley R G
Hawley T
Shulman M J
Traunecker A
Köhler G
Hozumi N
References (40)
40 references, click to expand
  1. Transfer of a cloned immunoglobulin light-chain gene to mutant hybridoma cells restores specific antibody production.
    Nature. 1983 Mar 24-30;302(5906):340-2 PMID: 6403868
  2. Correct transcription of a cloned mouse immunoglobulin gene in vivo.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):417-21 PMID: 6300842
  3. Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 PMID: 6304690
  4. High mutation frequency in DNA transfected into mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9 PMID: 6574469
  5. A tissue-specific transcription enhancer element is located in the major intron of a rearranged immunoglobulin heavy chain gene.
    Cell. 1983 Jul;33(3):717-28 PMID: 6409417
  6. Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA.
    Mol Cell Biol. 1983 Jul;3(7):1317-32 PMID: 6412070
  7. Deletions in immunoglobulin mu chains.
    EMBO J. 1982;1(5):555-63 PMID: 6329690
  8. Structural basis of antibody function.
    Annu Rev Immunol. 1983;1:87-117 PMID: 6399980
  9. Expression and regulation of immunoglobulin heavy chain gene transfected into lymphoid cells.
    EMBO J. 1983;2(8):1373-8 PMID: 10872333
  10. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  11. Isolation of high-molecular-weight DNA from mammalian cells.
    Eur J Biochem. 1973 Jul 2;36(1):32-8 PMID: 4200179
  12. Continuous cultures of fused cells secreting antibody of predefined specificity.
    Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
  13. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  14. Derivation of specific antibody-producing tissue culture and tumor lines by cell fusion.
    Eur J Immunol. 1976 Jul;6(7):511-9 PMID: 825377
  15. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  16. Recombinant DNA clones constructed from immunoglobulin kappa light chain messenger RNA.
    Nucleic Acids Res. 1978 Sep;5(9):3113-28 PMID: 100767
  17. A better cell line for making hybridomas secreting specific antibodies.
    Nature. 1978 Nov 16;276(5685):269-70 PMID: 714156
  18. The synthesis and processing of the messenger RNAs specifying heavy and light chain immunoglobulins in MPC-11 cells.
    Cell. 1978 Dec;15(4):1495-509 PMID: 103631
  19. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
    J Immunol. 1979 Oct;123(4):1548-50 PMID: 113458
  20. Intervening sequences divide the gene for the constant region of mouse immunoglobulin mu chains into segments, each encoding a domain.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):554-8 PMID: 6767239
  21. Synthesis of secreted and membrane-bound immunoglobulin mu heavy chains is directed by mRNAs that differ at their 3' ends.
    Cell. 1980 Jun;20(2):293-301 PMID: 6771018
  22. Two mRNAs with different 3' ends encode membrane-bound and secreted forms of immunoglobulin mu chain.
    Cell. 1980 Jun;20(2):303-12 PMID: 6771019
  23. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  24. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  25. Myeloma with multiple rearranged immunoglobulin kappa genes: only one kappa gene codes for kappa chains.
    Nucleic Acids Res. 1980 Oct 24;8(20):4681-7 PMID: 6255428
  26. Comparison of different rearranged immunoglobulin kappa genes of a myeloma by electronmicroscopy and restriction mapping of cloned DNA: implications for "allelic exclusion".
    Nucleic Acids Res. 1980 Oct 24;8(20):4689-707 PMID: 6255429
  27. Transforming DNA integrates into the host chromosome.
    Cell. 1981 Jan;23(1):29-39 PMID: 7214526
  28. Transcripts of the immunoglobulin C mu gene vary in structure and splicing during lymphoid development.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7400-4 PMID: 6784123
  29. Molecular cloning of an immunoglobulin kappa constant gene from NZB mouse.
    Gene. 1981 Mar;13(2):163-172 PMID: 6453743
  30. RNA splicing mutation in an aberrantly rearranged immunoglobulin lambda I gene.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):7019-23 PMID: 6171827
  31. The cloning and reintroduction into animal cells of a functional CAD gene, a dominant amplifiable genetic marker.
    Cell. 1981 Dec;27(2 Pt 1):267-77 PMID: 6277500
  32. Expression of mouse immunoglobulin genes in monkey cells.
    Nature. 1982 Jul 15;298(5871):286-8 PMID: 6283383
  33. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  34. Multiple immunoglobulin heavy-chain gene transcripts in Abelson murine leukemia virus-transformed lymphoid cell lines.
    Mol Cell Biol. 1982 Apr;2(4):386-400 PMID: 6810096
  35. Mutations affecting the structure and function of immunoglobulin M.
    Mol Cell Biol. 1982 Sep;2(9):1033-43 PMID: 6817080
  36. An immunoglobulin promoter region is unaltered by DNA rearrangement and somatic mutation during B-cell development.
    Nucleic Acids Res. 1982 Dec 11;10(23):7731-49 PMID: 6296789
  37. Mutant immunoglobulin genes have repetitive DNA elements inserted into their intervening sequences.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7425-9 PMID: 6296832
  38. Regulated expression of an immunoglobulin kappa gene introduced into a mouse lymphoid cell line.
    Proc Natl Acad Sci U S A. 1982 Dec;79(24):7862-5 PMID: 6818555
  39. Patterns of integration of DNA microinjected into cultured mammalian cells: evidence for homologous recombination between injected plasmid DNA molecules.
    Mol Cell Biol. 1982 Nov;2(11):1372-87 PMID: 6298598
  40. Immunoglobulin gene expression in transformed lymphoid cells.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):825-9 PMID: 6402777
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.20.6351
Published
1983-10-00
Pages
6351-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC394295
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.20.6351 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2