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PMID: 3264155 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A survey of the kinetic parameters of class C beta-lactamases. Cephalosporins and other beta-lactam compounds.

The Biochemical journal ·Vol. 255 ·No. 1 ·1988-10-01 ·Pages 123-9

Galleni M, Amicosante G, Frère JM

Abstract

Various cephalosporins, cefoxitin, moxalactam, imipenem and aztreonam were studied as substrates of six class C beta-lactamases. Nitrocefin, cephaloridine, cefazolin, cephalothin and cephalexin were good substrates, with kcat. values ranging from 27 to 5000 s-1. Cefuroxime, cefotaxime and cefoxitin exhibited low kcat. values (0.010-1.7 s-1) and low Km values, which suggested a rate-limiting deacylation. Imipenem and aztreonam were even poorer substrates (kcat. 2 x 10(-4)-3 x 10(-2) s-1) and, in the presence of a reporter substrate, behaved as transient inactivators. With moxalactam, biphasic kinetics were observed, indicating a possible rearrangement of the acyl-enzyme.

MeSH Terms
Aztreonam/metabolism Cephalosporins/metabolism Hydrolysis Imipenem/metabolism Kinetics Moxalactam/metabolism Substrate Specificity beta-Lactamases/classification,metabolism
Chemicals
Cephalosporins Imipenem beta-Lactamases Aztreonam Moxalactam
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Galleni M
Laboratoire d'Enzymologie, Université de Liège, Sart Tilman, Belgium.
Amicosante G
Frère J M
References (18)
18 references, click to expand
  1. The beta-lactamases of gram-negative bacteria and their possible physiological role.
    Adv Microb Physiol. 1973;9:31-88 PMID: 4581138
  2. A survey of the kinetic parameters of class C beta-lactamases. Penicillins.
    Biochem J. 1988 Oct 1;255(1):119-22 PMID: 3264154
  3. Purification and properties of a cephalosporinase from Enterobacter cloacae.
    Antimicrob Agents Chemother. 1980 Dec;18(6):853-7 PMID: 6972193
  4. Purification and properties of chromosomally mediated beta-lactamase from Citrobacter freundii GN7391.
    J Gen Microbiol. 1980 Dec;121(2):449-56 PMID: 6973612
  5. Isolation and properties of an inducible and a constitutive beta-lactamase from Pseudomonas aeruginosa.
    J Gen Microbiol. 1982 Jan;128(1):155-9 PMID: 6806437
  6. Interaction of azthreonam and related monobactams with beta-lactamases from gram-negative bacteria.
    Antimicrob Agents Chemother. 1982 Sep;22(3):414-20 PMID: 6982680
  7. The acyl-enzyme mechanism of beta-lactamase action. The evidence for class C Beta-lactamases.
    Biochem J. 1982 Nov 1;207(2):315-22 PMID: 6818947
  8. Kinetics and significance of the activity of the Sabath and Abrahams' beta-lactamase of Pseudomonas aeruginosa against cefotaxime and cefsulodin.
    J Antimicrob Chemother. 1983 Feb;11(2):169-79 PMID: 6300016
  9. Chromosomal beta-lactamases of Enterobacter cloacae are responsible for resistance to third-generation cephalosporins.
    Antimicrob Agents Chemother. 1983 Jun;23(6):918-25 PMID: 6351733
  10. beta-Lactamase inhibitors.
    Med Res Rev. 1983 Oct-Dec;3(4):341-82 PMID: 6358721
  11. Mechanism of inhibition of the PC1 beta-lactamase of Staphylococcus aureus by cephalosporins: importance of the 3'-leaving group.
    Biochemistry. 1985 Feb 12;24(4):903-10 PMID: 3873255
  12. The beta-lactamase of Enterobacter cloacae P99. Chemical properties, N-terminal sequence and interaction with 6 beta-halogenopenicillanates.
    Biochem J. 1985 May 15;228(1):241-8 PMID: 2988516
  13. 6-beta-Iodopenicillanate as a probe for the classification of beta-lactamases.
    Biochem J. 1986 Nov 1;239(3):575-80 PMID: 3030266
  14. Properties of a class C beta-lactamase from Serratia marcescens.
    Biochem J. 1986 Nov 1;239(3):581-6 PMID: 3548700
  15. Automated analysis of enzyme inactivation phenomena. Application to beta-lactamases and DD-peptidases.
    Biochem Pharmacol. 1987 Jul 15;36(14):2393-403 PMID: 3038122
  16. Structure-activity relationships in the beta-lactam family: an impossible dream.
    Biochem Pharmacol. 1988 Jan 1;37(1):125-32 PMID: 3276315
  17. Sensitivity of Escherichia coli to various beta-lactams is determined by the interplay of outer membrane permeability and degradation by periplasmic beta-lactamases: a quantitative predictive treatment.
    Mol Microbiol. 1987 Jul;1(1):29-36 PMID: 3330755
  18. Enzymatic and immunological characterization of a new cephalosporinase from Enterobacter aerogenes.
    Antimicrob Agents Chemother. 1977 Aug;12(2):201-5 PMID: 900918
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2550123
Published
1988-10-01
Pages
123-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135199
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2550123 PMC Full Text View journal details
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