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PMID: 3264154 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A survey of the kinetic parameters of class C beta-lactamases. Penicillins.

The Biochemical journal ·Vol. 255 ·No. 1 ·1988-10-01 ·Pages 119-22

Galleni M, Frère JM

Abstract

The interaction between six class C beta-lactamases and various penicillins has been studied. All the enzymes behaved in a very uniform manner. Benzylpenicillin exhibited relatively low kcat. values (14-75 s-1) but low values of Km resulted in high catalytic efficiencies [kcat./Km = 10 X 10(6)-75 X 10(6) M-1.s-1]. The kcat. values for ampicillin were 10-100-fold lower. Carbenicillin, oxacillin cloxacillin and methicillin were very poor substrates, exhibiting kcat. values between 1 x 10(-3) and 0.1 s-1. The Km values were correspondingly small. It could safely be hypothesized that, with all the tested substrates, deacylation was rate-limiting, resulting in acyl-enzyme accumulation.

MeSH Terms
Ampicillin/metabolism Carbenicillin/metabolism Cloxacillin/metabolism Isoenzymes/metabolism Kinetics Methicillin/metabolism Oxacillin/metabolism Penicillin G/metabolism Penicillins/metabolism Substrate Specificity beta-Lactamases/metabolism
Chemicals
Isoenzymes Penicillins Ampicillin beta-Lactamases Carbenicillin Cloxacillin Penicillin G Methicillin Oxacillin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Galleni M
Laboratoire d'Enzymologie, Université de Liège, Sart Tilman, Belgium.
Frère J M
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2550119
Published
1988-10-01
Pages
119-22
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1135198
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2550119 PMC Full Text View journal details
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