Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.

Nucleic acids research ·Vol. 16 ·No. 24 ·1988-12-23 ·Pages 11461-7

Bautsch W

Abstract

A macrorestriction map of the 780 kbp Mycoplasma mobile (ATCC 43663) genome was constructed. Linking fragments were identified on two-dimensional pulsed-field electrophoresis gels. Either complete double restriction digests or partial and complete single digests were separated in the first and second dimension, respectively. 19 restriction sites of four enzymes could be assigned to the map. These rapid methods do not require DNA probes and are applicable to the long-range restriction mapping of all genomes that yield resolvable patterns on two-dimensional gels.

MeSH Terms

Bacterial Proteins DNA, Bacterial/isolation & purification Deoxyribonuclease BamHI Deoxyribonucleases, Type II Site-Specific Electrophoresis, Gel, Two-Dimensional/methods Mycoplasma/genetics Restriction Mapping/methods

Chemicals

Bacterial Proteins DNA, Bacterial Deoxyribonuclease BamHI endodeoxyribonuclease MluI endodeoxyribonuclease NruI Deoxyribonucleases, Type II Site-Specific GTGCAC-specific type II deoxyribonucleases

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Bautsch W

Abteilung Biophysikalische Chemie, Medizinische Hochschule Hannover, FRG.

References (10)

10 references, click to expand

Genome size of mycoplasmal DNA.
Nature. 1969 Dec 20;224(5225):1209-10 PMID: 5358346
Analysis of Mycoplasma hyorhinis genome by use of restriction endonucleases and by electron microscopy.
J Bacteriol. 1982 May;150(2):788-94 PMID: 6279572
Separation of chromosomal DNA molecules from yeast by orthogonal-field-alternation gel electrophoresis.
Nucleic Acids Res. 1984 Jul 25;12(14):5647-64 PMID: 6379602
Isolation of a motile mycoplasma from fish.
J Gen Microbiol. 1984 Sep;130(9):2439-45 PMID: 6502137
A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci.
Nucleic Acids Res. 1988 Jul 11;16(13):6027-39 PMID: 2840640
A physical map of the Escherichia coli K12 genome.
Science. 1987 Jun 12;236(4807):1448-53 PMID: 3296194
Restriction endonucleases for pulsed field mapping of bacterial genomes.
Nucleic Acids Res. 1987 Aug 11;15(15):5985-6005 PMID: 2819819
Mapping genomic organization by field inversion and two-dimensional gel electrophoresis: application to the murine T-cell receptor gamma gene family.
Nucleic Acids Res. 1988 May 11;16(9):3863-75 PMID: 3375073
Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes.
Nucleic Acids Res. 1988 Jul 11;16(13):6015-25 PMID: 2840639
Electrophoretic separations of large DNA molecules by periodic inversion of the electric field.
Science. 1986 Apr 4;232(4746):65-8 PMID: 3952500

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/16.24.11461

Published

1988-12-23

Pages

11461-7

Language

English

Region

England

NLM ID

0411011

PMCID

PMC339058

Subset

External Links

PubMed source DOI: 10.1093/nar/16.24.11461 PMC Full Text View journal details

Analysis Services

Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques.

Contact

WeChat Official Account